- Clone
- 9E9 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- FcgRIV, Fcgr4, Fcrl3, CD16-2, FcgRIV, Fcgr3a, FcgammaRIV, 4833442P21Rik, Fc gamma RIV
- Isotype
- Armenian Hamster IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![9E9_BV421_CD16point2_Antibody_112816 9E9_BV421_CD16point2_Antibody_112816](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/9E9_BV421_CD16point2_Antibody_112816.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse bone marrow cells were stained with CD16.2 (clone 9E9) Brilliant Violet 421™ (filled histogram) or Armenian hamster IgG Brilliant Violet 421™ isotype control (open histogram). Data shown was gated on the myeloid population.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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149521 | 50 µg | 264 CHF |
FcγRIV, also known as CD16.2, is an intermediate-affinity activating receptor for IgG2a and IgG2b. CD16.2 is the mouse homolog of human FcγRIIIA. CD16.2 is a low-affinity IgE receptor for all allotypes and the ligation of FcγRIV by antigen-IgE immune complexes promotes macrophage-mediated phagocytosis and is involved in lung inflammation.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- FCγR4 —EC domain fusion with IgG1 Fc
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
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Violet Laser (405 nm)
- Application Notes
-
Additional reported applications (for the relevant formats of this clone) include: blocking of Fc?RIV function1 and inhibition of immune complex binding1,2. The LEAF™ or Ultra-LEAF™ purified antibody (Endotoxin < EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (contact our custom solutions team).
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Application References
(PubMed link indicates BioLegend citation) -
- Mancardi DA, et al. 2008. J. Clin. Invest 118:3738. (FC, Block)
- Nimmerjahn F, et al. 2005. Immunity 23:41.
- RRID
-
AB_2632892 (BioLegend Cat. No. 149521)
Antigen Details
- Structure
- 22.4 kD
- Distribution
-
Expressed on peripheral blood leukocytes, spleen, colon, bone marrow, and the intestine
- Function
- Activating receptor for IgG2a and IgG2b via association with an ITAM bearing accessory.
- Ligand/Receptor
- IgG2a and IgG2b
- Cell Type
- B cells, Leukocytes
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Fc Receptors
- Antigen References
-
- Mechetina LV, et al. 2002. Immunogenetics. 54:463-8.
- Nimmerjahn F, et al. 2005. Immunity. 23:41-51.
- Seeling M, et al. 2013. Proc. Natl. Acad. Sci. 110:10729-34.
- Gene ID
- 246256 View all products for this Gene ID
- UniProt
- View information about CD16.2 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- What is the F/P ratio range of our BV421™ format antibody reagents?
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It is lot-specific. On average it ranges between 2-4.
Other Formats
View All CD16.2 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse CD16.2 (FcγRIV) | 9E9 | FC,IHC-F |
PE anti-mouse CD16.2 (FcγRIV) | 9E9 | FC |
APC anti-mouse CD16.2 (FcγRIV) | 9E9 | FC |
Biotin anti-mouse CD16.2 (FcγRIV) | 9E9 | FC |
PE/Cyanine7 anti-mouse CD16.2 (FcγRIV) | 9E9 | FC |
PE/Dazzle™ 594 anti-mouse CD16.2 (FcγRIV) | 9E9 | FC |
FITC anti-mouse CD16.2 (FcγRIV) | 9E9 | FC |
Brilliant Violet 421™ anti-mouse CD16.2 (FcγRIV) | 9E9 | FC |
Alexa Fluor® 488 anti-mouse CD16.2 (FcγRIV) | 9E9 | FC |
Alexa Fluor® 647 anti-mouse CD16.2 (FcγRIV) | 9E9 | FC |
Brilliant Violet 711™ anti-mouse CD16.2 (FcγRIV) | 9E9 | FC |
Brilliant Violet 785™ anti-mouse CD16.2 (FcγRIV) | 9E9 | FC |
Brilliant Violet 650™ anti-mouse CD16.2 (FcγRIV) | 9E9 | FC |
Brilliant Violet 510™ anti-mouse CD16.2 (FcγRIV) | 9E9 | FC |
APC/Cyanine7 anti-mouse CD16.2 (FcγRIV) | 9E9 | FC |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD16.2 (FcγRIV)
C57BL/6 bone marrow cells were stained with purified CD16.2 ... C57BL/6 mouse frozen spleen section was fixed with 4% parafo... C57BL/6 mouse frozen intestine section was fixed with 4% par... -
PE anti-mouse CD16.2 (FcγRIV)
C57BL/6 bone marrow cells were stained with CD16.2 (clone 9E... -
APC anti-mouse CD16.2 (FcγRIV)
C57BL/6 bone marrow cells were stained with CD16.2 (clone 9E... -
Biotin anti-mouse CD16.2 (FcγRIV)
C57BL/6 mouse bone marrow cells were stained with biotinylat... -
PE/Cyanine7 anti-mouse CD16.2 (FcγRIV)
C57BL/6 mouse bone marrow cells were stained with CD16.2 (cl... -
PE/Dazzle™ 594 anti-mouse CD16.2 (FcγRIV)
C57BL/6 mouse bone marrow cells were stained with CD16.2 (cl... -
FITC anti-mouse CD16.2 (FcγRIV)
C57BL/6 mouse bone marrow cells were stained with CD16.2 (cl... -
Brilliant Violet 421™ anti-mouse CD16.2 (FcγRIV)
C57BL/6 mouse bone marrow cells were stained with CD16.2 (cl... -
Alexa Fluor® 488 anti-mouse CD16.2 (FcγRIV)
C57BL/6 bone marrow cells were stained with Alexa Fluor... -
Alexa Fluor® 647 anti-mouse CD16.2 (FcγRIV)
C57BL/6 bone marrow cells were stained with CD16.2 (clone 9E... -
Brilliant Violet 711™ anti-mouse CD16.2 (FcγRIV)
C57BL/6 mouse bone marrow cells were stained with CD16.2 (cl... -
Brilliant Violet 785™ anti-mouse CD16.2 (FcγRIV)
C57BL/6 mouse bone marrow cells were stained with CD16.2 (cl... -
Brilliant Violet 650™ anti-mouse CD16.2 (FcγRIV)
C57BL/6 mouse bone marrow cells were stained with CD16.2 (cl... -
Brilliant Violet 510™ anti-mouse CD16.2 (FcγRIV)
C57BL/6 mouse bone marrow cells were stained with CD16.2 (cl... -
APC/Cyanine7 anti-mouse CD16.2 (FcγRIV)
C57BL/6 myeloid cells were stained with anti-mouse CD16.2 (c...
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