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Brilliant Violet 421™ anti-mouse CD192 (CCR2) Antibody

Pricing & Availability
Clone
SA203G11 (See other available formats)
Regulatory Status
RUO
Other Names
Ccr2, Ckr2, Ccr2a, Ccr2b
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
SA203G11_BV421_CD192_Antibody_1_FC_031016
C57BL/6 bone marrow was stained with Ly-6G APC/Cyanine7, CD11b PE, and CD192 (clone SA203G11) Brilliant Violet 421™ (top) or rat IgG2b, κ Brilliant Violet 421™ isotype control (bottom). Dot plots are gated on Ly-6G- cells.
  • SA203G11_BV421_CD192_Antibody_1_FC_031016
    C57BL/6 bone marrow was stained with Ly-6G APC/Cyanine7, CD11b PE, and CD192 (clone SA203G11) Brilliant Violet 421™ (top) or rat IgG2b, κ Brilliant Violet 421™ isotype control (bottom). Dot plots are gated on Ly-6G- cells.
  • SA203G11_BV421_CD192_Antibody_2_FC_031016
Compare all formats See Brilliant Violet 421™ spectral data
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150605 50 µg 292 CHF
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Description

CD192, also known as CCR2, is a 42 kD G-protein coupled receptor that is associated with bone marrow homeostasis. Specifically, CD192 mediates monocyte chemotaxis and acts as a receptor for monocyte chemoattractant protein 1 (MCP-1). CD192 is primarily expressed on monocytes and macrophages, with some expression on basophils. It is involved in monocyte infiltration in inflammatory diseases such as rheumatoid arthritis and cancer.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CCR2 transfectants.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Product Citations
  1. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
  2. Edwards CL, et al. 2023. J Clin Invest. 133: . PubMed
  3. Drummer C, et al. 2023. Front Immunol. 14:1113883. PubMed
  4. Silva BM, et al. 2023. J Clin Invest. 133:. PubMed
  5. Gonalves S, et al. 2021. Cell Reports. 34(11):108860. PubMed
  6. Ferrere G, et al. 2021. JCI Insight. 6:. PubMed
  7. Yang F, et al. 2020. Cancer Res. 80:3677. PubMed
  8. Monaghan KL, et al. 2020. J Vis Exp. . PubMed
  9. Yao Y, et al. 2018. Cell. 175:1634. PubMed
  10. Jung SH, et al. 2022. Nat Commun. 13:4580. PubMed
  11. Wong NR, et al. 2021. Immunity. 54:2072. PubMed
  12. Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
  13. Gong P, et al. 2022. Cell Death Discov. 8:466. PubMed
  14. Sharma R, et al. 2021. J Neuroinflammation. 72:18. PubMed
  15. Bosteels C, et al. 2020. Immunity. 52(6):1039-1056.e9.. PubMed
RRID
AB_2571913 (BioLegend Cat. No. 150605)

Antigen Details

Structure
G-protein-coupled receptor, 7-transmembrane protein, 42 kD.
Distribution

Monocytes/macrophages and basophils.

Function
Mediator of monocyte chemotaxis and bone marrow homeostasis.
Ligand/Receptor
MCP-1, Ccl2, Ccl7, and Ccl13.
Cell Type
Basophils, Macrophages, Monocytes
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Mack M, et al. 2001. J. Immunol. 166:4697.
2. Dutta P, et al. 2015. Cell Stem Cell. 16:477.
3. Li L, et al. 2008. Kidney Int. 74:1526.

Gene ID
12772 View all products for this Gene ID
UniProt
View information about CD192 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 03.11.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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