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Brilliant Violet 421™ anti-mouse/human Mac-2 (Galectin-3) Antibody

Pricing & Availability
Clone
M3/38 (See other available formats)
Regulatory Status
RUO
Other Names
Galectin-3, Mac-2, Gal-3, RL-29, galactose-specific lectin 3, CBP-35, L-34, εBP
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
M3-38_BV421_Mac-2_Antibody_102820
C57BL/6 mouse frozen colon section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS plus 5% rat serum for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of Mac-2 (Galectin-3) (clone M3/38) Brilliant Violet 421™ (blue) and co-stained with 5 µg/mL anti-mouse/human CD324 (E-Cadherin) Alexa Fluor® 594 (green) overnight at 4°C. DRAQ5™ (red) was used to counterstain the nucleus. The image was captured with a 10X objective.
  • M3-38_BV421_Mac-2_Antibody_102820
    C57BL/6 mouse frozen colon section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS plus 5% rat serum for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of Mac-2 (Galectin-3) (clone M3/38) Brilliant Violet 421™ (blue) and co-stained with 5 µg/mL anti-mouse/human CD324 (E-Cadherin) Alexa Fluor® 594 (green) overnight at 4°C. DRAQ5™ (red) was used to counterstain the nucleus. The image was captured with a 10X objective.
Compare all formats See Brilliant Violet 421™ spectral data
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125416 100 µg 435 CHF
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Description

Galectins, a family of carbohydrate binding proteins (lectins) have been implicated in inflammation and cancer. All galectins bind lactose and other beta-galactosides but differ in their affinity for more complex saccharides.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Raised against galectin-3 of mouse origin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-F - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 5.0 - 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections3-6, Western blotting2, immunoprecipitation1,2, immunofluorescence7,8, and ELISA9.

Clone M3/38 has been reported to recognize residues 48-100 in the amino-terminal domain of galectin-37.

Application References

(PubMed link indicates BioLegend citation)
  1. Ho MK. and Springer TA. 1982. J. Immunol. 128:1221. (FC, IP)
  2. Rosenberg I, et al. 1991. J. Biol. Chem. 266:18731. (WB, IP)
  3. Evans CE, et al. 2010. Arterioscler Vasc Biol. (IHC) PubMed
  4. Jacob N, et al. 2011. J. Immunol. 186:4984. (IHC) PubMed
  5. Li X, et al. 2011. Am J Physiol Heart Circ Physiol. 301:1932. (IHC) PubMed
  6. Chao C, et al. 2012. Clin Cancer Res. 18:4702. (IHC) PubMed
  7. Melo FH, et al. 2011. PLoS One. 6:e29313. (IF)
  8. Usategui A, et al. 2013. Ann Rheum Dis. 72:2018. PubMed (IF)
  9. Mey A, et al. 1996. J. Immunol. 156:1572. (ELISA)
  10. Reales E, et al. 2015. J Cell Sci. 128:2261. PubMed
Product Citations
  1. Bussi C, et al. 2022. Nat Commun. 13:7338. PubMed
  2. Gordon-Alonso M,et al. 2017. Nat. Commun. 10.1038/s41467-017-00925-6. PubMed
RRID
AB_2566686 (BioLegend Cat. No. 125416)

Antigen Details

Biology Area
Cell Biology, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules
Antigen References

1. Ho MK. and Springer TA. 1982. J. Immunol. 128:1221.
2. Rosenberg I, et al. 1991. J. Biol. Chem. 266:18731.

Gene ID
16854 View all products for this Gene ID 3958 View all products for this Gene ID
UniProt
View information about Mac-2 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 2    Revision Date: 10.28.2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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