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Brilliant Violet 421™ anti-mouse Ki-67 Antibody

Pricing & Availability
Clone
16A8 (See other available formats)
Regulatory Status
RUO
Other Names
KIA, proliferation-related Ki-67 antigen
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
16A8_BV421_Ki-67_Antibody_FC_111113
Con A+IL-2-stimulated (3 days) C57BL/6 mouse splenocytes were fixed and permeabilized with 70% ethanol, and then stained with Ki-67 (clone 16A8) Brilliant Violet 421™ (filled histogram) or rat IgG2a, κ Brilliant Violet 421™ isotype control (open histogram).
  • 16A8_BV421_Ki-67_Antibody_FC_111113
    Con A+IL-2-stimulated (3 days) C57BL/6 mouse splenocytes were fixed and permeabilized with 70% ethanol, and then stained with Ki-67 (clone 16A8) Brilliant Violet 421™ (filled histogram) or rat IgG2a, κ Brilliant Violet 421™ isotype control (open histogram).
Compare all formats See Brilliant Violet 421™ spectral data
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652411 50 µg 286 CHF
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Description

The nuclear protein Ki-67 was first identified by the monoclonal antibody Ki-67, which was generated by immunizing mice with nuclei of the L428 Hodgkin lymphoma cell line. Ki-67 protein plays an essential role in ribosomal RNA transcription and cell proliferation. Expression of Ki-67 occurs during G1, S, G2, and M phase, while in G0 phase the Ki-67 protein is not detectable. Ki-67 is strongly expressed in proliferating cells and has been reported as a prognostic marker in various tumors.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli expressed partial mouse Ki-67 recombinant protein, 1816-2163 aa.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by our Ki-67 staining protocol below. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Medina-Reyes EI, et al. 2015. Environ Res. 136:424. PubMed
  2. Guillaumond F, et al. 2015. PNAS. 112:2473. PubMed
  3. Sharma SK, et al. 2015. J Immunol. 194:5529. PubMed
  4. Rodero MP, et al. 2014. J. Invest. Dermatol. 7:1991-7. PubMed
Product Citations
  1. Broadfield LA, et al. 2022. Mol Metab. 61:101498. PubMed
  2. Li W, et al. 2023. Bioact Mater. 22:34. PubMed
  3. Weng S, et al. 2022. Front Immunol. 13:1025931. PubMed
  4. Lin YH 2023. Immunity. 56(1):207-223.e8. PubMed
  5. Fiege JK, et al. 2021. Cell Host Microbe. 29:1815. PubMed
  6. Lee K, et al. 2023. JCI Insight. 8:. PubMed
  7. Piper CJM, et al. 2020. Cell Reports. 29(7):1878-1892.e7.. PubMed
  8. Choi H et al. 2019. Cell Rep. 27(3):806-819 . PubMed
  9. Montes de Oca R, et al. 2021. Mol Cancer Ther. 20:1941. PubMed
  10. Wang G, et al. 2021. Nat Commun. 12:5733. PubMed
  11. Yang W, et al. 2020. Mucosal Immunol. 13:788. PubMed
  12. Nagai Y, et al. 2019. Front Immunol. 10:174. PubMed
  13. Dai B, et al. 2022. Theranostics. 12:7603. PubMed
  14. Prasad M, et al. 2021. Front Immunol. 12:644483. PubMed
  15. Zhang H, et al. 2020. Cancer Cell. 37(1):37-54.e9.. PubMed
  16. MacDonald A, et al. 2021. Front Immunol. 12:755995. PubMed
  17. Ma X, et al. 2021. Cell Metabolism. 33(5):1001-1012.e5. PubMed
  18. Ma X et al. 2019. Cell Metab. 30(1):143-156 . PubMed
  19. Yu X, et al. 2019. Nat Commun. 10:574. PubMed
  20. Yao Y, et al. 2018. Cell. 175:1634. PubMed
  21. Koelwyn GJ, et al. 2020. Nat Med. 1452:26. PubMed
  22. Lehrke MJ, et al. 2021. Elife. 10:. PubMed
  23. Li N, et al. 2022. Nat Commun. 13:7281. PubMed
  24. Rustenhoven J, et al. 2021. Cell. 184(4):1000-1016.e27. PubMed
  25. Fedele C, et al. 2021. J Exp Med. 218: . PubMed
RRID
AB_2562663 (BioLegend Cat. No. 652411)

Antigen Details

Structure
325 kD protein containing a forkhead-associated domain (FHA) and 13 tandem repeats
Distribution

Nucleus and chromosome

Function
Required for cell cycle progression and proliferation
Interaction
Interacts with KIF15; binds to MKI67IP through FHA domain
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Starborg M, et al. 1996. J. Cell. Sci. 109:143.
2. Byeon IJ, et al. 2005. Nat. Struct. Mol. Biol. 12:987.
3. Yerushalmi R, et al. 2010. Lancet. Oncol. 11:174.
4. Beltrami AP, et al. 2001. N. Engl. J. Med. 344:1750.
5. Sachsenberg N, et al. 1998. J. Exp. Med. 187:1295.
6. Nagy Z, et al. 1997. Acta. Neuropathol. 93:294.

Gene ID
17345 View all products for this Gene ID
UniProt
View information about Ki-67 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 5    Revision Date: 01.08.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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