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Brilliant Violet 421™ anti-mouse TLT-2 Antibody

Pricing & Availability
Clone
MIH47 (See other available formats)
Regulatory Status
RUO
Other Names
TREML2, TREM-like transcript 2
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
MIH47_BV-421_TLT-2_FC_082624
Mouse TLT-2-transfected cells were stained with anti-mouse TLT-2 (clone MIH47) Brilliant Violet 421™ (filled histogram) or rat IgG2a, κ Brilliant Violet 421™ isotype control (open histogram).
  • MIH47_BV-421_TLT-2_FC_082624
    Mouse TLT-2-transfected cells were stained with anti-mouse TLT-2 (clone MIH47) Brilliant Violet 421™ (filled histogram) or rat IgG2a, κ Brilliant Violet 421™ isotype control (open histogram).
See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability Save
136605 50 µg 270 CHF
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Description

Triggering receptor expressed on myeloid cell-like transcript 2 (TLT-2, TREML2) is a type I transmembrane protein in TREM family. It consists of a single extracellular Ig-like domain of the V-type, a transmembrane domain, and a short cytoplasmic domain. TLT-2 is expressed on myeloid , B cells , and macrophages. It was also found to express on CD8+ T cells and activated CD4+ T cells. It regulates innate immune response based on its expression profile, costimulates the activation of T cells. Transduction of TLT-2 into T cells resulted in enhanced IL-2 and IFN-gamma production. It was reported that B3-H7 is a receptor of TLT-2. Blockade of the B7-H3/ TLT-2 pathway with monoclonal antibodies against B7-H3 or TLT-2 efficiently inhibited hypersensitivity response. Further studies are needed to confirm the ligand.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse TLT-2 Ig fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Hashiguchi M, et al. 2008. Proc. Nat. Acad. Sci. U. S. A. 105(30):10495

Antigen Details

Structure
A type I transmembrane protein in TREM family, consists of a single extracellular Ig-like domain of the V-type, a transmembrane domain, and a short cytoplasmic domain.
Distribution

Expressed on myeloid cells, B cells, macrophages, CD8+ T cells, and activated CD4+ T cells.

Function
Regulate innate immune response, IL-2, IFN-gamma production, and hypersensitivity response.
Cell Type
B cells, Macrophages, T cells
Biology Area
Immunology, Innate Immunity
Antigen References

1. King RG, et al. 2006. J. Immunol. 176:6012
2. Ford JW, et al. 2009. Curr Opin Immunol. 21(1):38
3. Leitner J, et al. 2009. Eur. J. Immunol. 39(7):1754

Gene ID
328833 View all products for this Gene ID
UniProt
View information about TLT-2 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 08.19.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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