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Brilliant Violet 421™ anti-mouse TSLPR (TSLP-R) Antibody

Pricing & Availability
Clone
22H9 (See other available formats)
Regulatory Status
RUO
Other Names
Thymic stromal lymphopoietin protein receptor, cytokine receptor-like 2, CRL2, CRLF2, IL-XR, TSLP-R
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
22H9_BV421_TSLPR_Antibody_1_060117
C57BL/6 bone marrow cells were stained with CD45R/B220 FITC followed by TSLPR (clone 22H9 top) Brilliant Violet 421™ or rat IgG2a, κ Brilliant Violet 421™ isotype control (bottom), Data shown is gated on lymphoid population.
  • 22H9_BV421_TSLPR_Antibody_1_060117
    C57BL/6 bone marrow cells were stained with CD45R/B220 FITC followed by TSLPR (clone 22H9 top) Brilliant Violet 421™ or rat IgG2a, κ Brilliant Violet 421™ isotype control (bottom), Data shown is gated on lymphoid population.
  • 22H9_BV421_TSLPR_Antibody_2_060117
Compare all formats See Brilliant Violet 421™ spectral data
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151807 50 µg 292 CHF
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Description

TSLP-R, also known as the thymic stromal lymphopoietin protein receptor, cytokine receptor-like 2, CRL2, and IL-XR, is a 40kD type I membrane receptor that forms a functional heterodimeric complex with IL-7R and binds with TSLP. TSLPR is required for proper protein folding and a box1 motif important for association with the JAKs. TSLP-R is expressed in myeloid cells, dendritic cells, B cells and activated monocytes. It is weakly expressed in T cells. Expression has also been reported in heart, skeletal, muscle, and kidney tissues. Ligand receptor interactions have been implicated in the development of the hematopoietic system, dendritic cell maturation, and the maintenance and polarization of human Th2 memory T cells in allergy diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
TSLP Receptor Protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. 


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Isaksen DE, et al. 2002. J. Immunol. 168:3288. (FC)
  2. Bell BD, et al. 2013. Nat. Immunol. 14:364. (FC)
  3. Iseki M, et al. 2012. International Immunology 24:183. (FC)
  4. Astrakhan A, et al. 2007. Nature Immunology 8:522. (FC)
Product Citations
  1. Wiesner DL, et al. 2020. Cell Host Microbe. 614:27. PubMed
RRID
AB_2687305 (BioLegend Cat. No. 151807)

Antigen Details

Structure
Type I membrane receptor, forms functional heterodimeric complex with IL-7R to bind TSLP. Contains WSXWS motif required for proper protein folding and a box1 motif that is important for association with the JAKs.
Distribution

Expressed preferentially in myeloid cells including dendritic cells, B cells, activated monocytes, weakly expressed in T cells. Also expressed in heart, skeletal, muscle, and kidney cells.

Function
Binds to TSLP to stimulate cell proliferation.
Ligand/Receptor
TSLP
Cell Type
Dendritic cells, B cells, Monocytes
Biology Area
Immunology
Molecular Family
Cytokine/Chemokine Receptors
Antigen References

1. Reche PA, et al. 2001. J. Immunol. 167:336.
2. Tonozuka Y, et al. 2001. Cytogenet. Cell Genet. 93:23.
3. Zhang W, et al. 2001. Biochem. Biophys. Res. Commun. 281:878.
4. Wang YH, et al. 2006. Immunity 24:827.

Gene ID
57914 View all products for this Gene ID
UniProt
View information about TSLPR on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 0    Revision Date: 06.05.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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