Brilliant Violet 510™ anti-human CD1d Antibody

Product Details

Verified Reactivity

Human

Reported Reactivity

Cynomolgus

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Human CD1d-Fc fusion

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

Additional reported application (for the relevant formats) include: immunohistochemical staining of frozen tissue sections¹, Western blotting^1,2, and induction of IL-12 production by crosslinking of CD1d³.

Application References

(PubMed link indicates BioLegend citation)

1. Exley M, et al. 2000. Immunology 100:37. (IHC, WB)
2. Durante-Mangoni E, et al. 2004. J. Immunol. 173:2159. (WB)
3. Yue SC, et al. 2005. P. Natl. Acad. Sci. USA 102:11811. (Stim)

Product Citations

1. Munteanu AN, et al. 2019. Exp Ther Med. 18:1693. PubMed

RRID

AB_2563190 (BioLegend Cat. No. 350314)

Antigen Details

Structure

MHC-like, type I transmembrane protein, member of the CD1 family and the immunoglobulin superfamily

Distribution

Expressed by cortical thymocytes, monocytes, macrophages, dendritic cells, B cells (highly expressed on marginal zone B cells in particular), and in some non-lymphoid cells

Function

Present lipid antigens to iNKT cells

Ligand/Receptor

When loaded with a lipid, is recognized by the Vα14i TCR from iNKT cells

Cell Type

B cells, Dendritic cells, Macrophages, Monocytes, Thymocytes

Biology Area

Immunology, Innate Immunity

Molecular Family

CD Molecules, MHC Antigens, TCRs

Antigen References

1. Koch M, et al. 2005. Nat. Immunol. 6:819.
2. Liu X, et al. 2010. P. Natl. Acad. Sci. USA 107:13010.
3. Zeissig S, et al. 2010. J. Clin. Invest. 120:2889.
4. Teige A, et al. 2010. J. Immunol. 185:345.

Gene ID

912 View all products for this Gene ID

UniProt

View information about CD1d on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

Other Formats

Certificate of Analysis