- Clone
- RMG1-1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Immunoglobulin G1
- Isotype
- Rat IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![RMG1-1_BV510_IgG1_Antibody_063017 RMG1-1_BV510_IgG1_Antibody_063017](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/RMG1-1_BV510_IgG1_Antibody_063017.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
-
Human peripheral blood lymphocytes were stained with purified anti-human CD3 (clone UCHT1, mouse IgG1 isotype, filled histogram) or mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG1 (clone RMG1-1) Brilliant Violet 510™.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
406621 | 50 µg | 248 CHF |
The RMG1-1 monoclonal antibody reacts with immunoglobulin G1 (IgG1) in all tested mouse haplotype (Igh-a and b). It does not react with other isotypes. The RMG1-1 monoclonal antibody may be used as a primary or secondary reagent for ELISA or immunofluorescent analysis.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse Ig cocktail
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
-
Application References
(PubMed link indicates BioLegend citation) - Product Citations
-
- RRID
-
AB_2687344 (BioLegend Cat. No. 406621)
Other Formats
View All IgG1 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
PerCP/Cyanine5.5 anti-mouse IgG1 | RMG1-1 | FC |
Purified anti-mouse IgG1 | RMG1-1 | ELISA,FC |
Biotin anti-mouse IgG1 | RMG1-1 | ELISA,IHC-F |
FITC anti-mouse IgG1 | RMG1-1 | FC |
PE anti-mouse IgG1 | RMG1-1 | FC |
APC anti-mouse IgG1 | RMG1-1 | FC |
PE/Cyanine7 anti-mouse IgG1 | RMG1-1 | FC |
Brilliant Violet 421™ anti-mouse IgG1 | RMG1-1 | FC |
Alexa Fluor® 647 anti-mouse IgG1 | RMG1-1 | FC,IHC-F,IHC-P |
APC/Cyanine7 anti-mouse IgG1 | RMG1-1 | FC |
Brilliant Violet 510™ anti-mouse IgG1 | RMG1-1 | FC |
Alexa Fluor® 488 anti-mouse IgG1 | RMG1-1 | FC |
APC/Fire™ 750 anti-mouse IgG1 | RMG1-1 | FC |
PE/Dazzle™ 594 anti-mouse IgG1 | RMG1-1 | FC |
Brilliant Violet 650™ anti-mouse IgG1 | RMG1-1 | FC |
Alexa Fluor® 700 anti-mouse IgG1 | RMG1-1 | FC |
Alexa Fluor® 594 anti-mouse IgG1 | RMG1-1 | IHC-P |
TotalSeq™-A1167 anti-mouse IgG1 | RMG1-1 | PG |
TotalSeq™-C1167 anti-mouse IgG1 | RMG1-1 | PG |
Customers Also Purchased
![](https://d1spbj2x7qk4bg.cloudfront.net/Files//Images/media_assets/products/product_images/90_PB_040810.jpg)
![](https://d1spbj2x7qk4bg.cloudfront.net/Files//Images/media_assets/products/product_images/RMG1-1_PE_IgG1_Antibody_071217.jpg)
![](https://d1spbj2x7qk4bg.cloudfront.net/Files//Images/media_assets/products/product_images/43A3_APC_050707.jpg)
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
PerCP/Cyanine5.5 anti-mouse IgG1
Human peripheral blood lymphocytes were stained with purifie... -
Purified anti-mouse IgG1
-
Biotin anti-mouse IgG1
-
FITC anti-mouse IgG1
-
PE anti-mouse IgG1
Human peripheral blood lymphocytes were stained with (filled... -
APC anti-mouse IgG1
Human peripheral blood lymphocytes were stained with purifie... -
PE/Cyanine7 anti-mouse IgG1
Human peripheral blood lymphocytes were stained with purifie... -
Brilliant Violet 421™ anti-mouse IgG1
Human peripheral blood lymphocytes were stained with purifie... -
Alexa Fluor® 647 anti-mouse IgG1
Human peripheral blood lymphocytes were stained with purifie... FVB/N mouse frozen thymus section was fixed with 4% paraform... IHC staining of Alexa Fluor® 647 anti-mouse IgG1 on formalin... -
APC/Cyanine7 anti-mouse IgG1
Human peripheral blood lymphocytes were stained with purifie... -
Brilliant Violet 510™ anti-mouse IgG1
Human peripheral blood lymphocytes were stained with purifie... -
Alexa Fluor® 488 anti-mouse IgG1
Human peripheral blood lymphocytes were stained with (filled... -
APC/Fire™ 750 anti-mouse IgG1
Human peripheral blood lymphocytes were stained with purifi... -
PE/Dazzle™ 594 anti-mouse IgG1
Human peripheral blood lymphocytes were stained with (filled... -
Brilliant Violet 650™ anti-mouse IgG1
Human peripheral blood lymphocytes were stained with purifie... -
Alexa Fluor® 700 anti-mouse IgG1
Human peripheral blood lymphocytes were stained with (filled... -
Alexa Fluor® 594 anti-mouse IgG1
Human paraffin-embedded tonsil tissue slices were prepared w... -
TotalSeq™-A1167 anti-mouse IgG1
-
TotalSeq™-C1167 anti-mouse IgG1
Follow Us