Brilliant Violet 570 anti-mouse CD3 Antibody anti-CD3

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Clone: 17A2 (See other available formats)
Regulatory Status: RUO
Other Names: T cell antigen receptor complex, T3
Isotype: Rat IgG2b, κ
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Product Citations: publications

17A2_BV570_1_072011 — C57BL/6 mouse splenocytes were stained with CD19 APC and CD3 (clone 17A2 Brilliant Violet 570™ (top) or rat IgG2b, κ Brilliant Violet 570™ isotype control (bottom).

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100225

125 µL

209 CHF

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Description

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: γδTCR-positive T-T hybridoma D1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 570™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 570™ excites at 405 nm and emits at 570 nm. The bandpass filter 585/42 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 570™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported application (for relevant formats) include: spatial biology (IBEX)^1,2.
RRID: AB_10900444 (BioLegend Cat. No. 100225)

Antigen Details

Structure: Ig superfamily, CD3/TCR, 20 kD
Distribution: Thymocytes (differentiation dependent), mature T cells, NK-T cells
Function: Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor: Peptide antigen/MHC-complex
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.
Gene ID: 12502 View all products for this Gene ID
UniProt: View information about CD3 on UniProt.org

Documentation

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Cell Surface Flow Cytometry Staining Protocol

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Other Formats

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Description	Clone	Applications
FITC anti-mouse CD3	17A2	FC
PE anti-mouse CD3	17A2	FC
Purified anti-mouse CD3	17A2	FC,IHC-F,IP,ICC
Alexa Fluor® 647 anti-mouse CD3	17A2	FC,IHC-F,3D IHC,SB
Alexa Fluor® 488 anti-mouse CD3	17A2	FC,IHC-F,3D IHC
Pacific Blue™ anti-mouse CD3	17A2	FC
Alexa Fluor® 700 anti-mouse CD3	17A2	FC
PerCP/Cyanine5.5 anti-mouse CD3	17A2	FC
PE/Cyanine7 anti-mouse CD3	17A2	FC
APC/Cyanine7 anti-mouse CD3	17A2	FC
Brilliant Violet 421™ anti-mouse CD3	17A2	FC,ICC
Brilliant Violet 570™ anti-mouse CD3	17A2	FC
Brilliant Violet 650™ anti-mouse CD3	17A2	FC
Brilliant Violet 785™ anti-mouse CD3	17A2	FC
Brilliant Violet 510™ anti-mouse CD3	17A2	FC
APC anti-mouse CD3	17A2	FC
Ultra-LEAF™ Purified anti-mouse CD3	17A2	FC,IHC-F,IP,ICC
Brilliant Violet 605™ anti-mouse CD3	17A2	FC
Alexa Fluor® 594 anti-mouse CD3	17A2	IHC-F,FC,3D IHC,SB
Brilliant Violet 711™ anti-mouse CD3	17A2	FC
Biotin anti-mouse CD3	17A2	FC,IHC-F
PE/Dazzle™ 594 anti-mouse CD3	17A2	FC
APC/Fire™ 750 anti-mouse CD3	17A2	FC
Brilliant Violet 750™ anti-mouse CD3	17A2	FC
TotalSeq™-A0182 anti-mouse CD3	17A2	PG
TotalSeq™-B0182 anti-mouse CD3	17A2	PG
Spark Blue™ 550 anti-mouse CD3	17A2	FC
Spark NIR™ 685 anti-mouse CD3	17A2	FC
TotalSeq™-C0182 anti-mouse CD3	17A2	PG
APC/Fire™ 810 anti-mouse CD3	17A2	FC
PE/Fire™ 640 anti-mouse CD3	17A2	FC
Spark YG™ 570 anti-mouse CD3	17A2	IHC-F
PE/Fire™ 700 anti-mouse CD3	17A2	FC
PE/Cyanine5 anti-mouse CD3	17A2	FC
Spark Blue™ 574 anti-mouse CD3 Antibody	17A2	FC
Spark Violet™ 423 anti-mouse CD3	17A2	FC
PE/Fire™ 810 anti-mouse CD3	17A2	FC
Spark Red™ 718 anti-mouse CD3	17A2	FC
Spark UV™ 387 anti-mouse CD3	17A2	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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