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Brilliant Violet 570™ Streptavidin

Pricing & Availability
Regulatory Status
RUO
Other Names
SAv-Brilliant Violet 570™
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Product Citations
publications
SAV_BV570_091611
Human peripheral blood lymphocytes were stained with biotinylated CD3 (clone UCHT1) (filled histogram) or mouse IgG1 isotype control (open histogram), followed with Sav-Brilliant Violet 570™.
  • SAV_BV570_091611
    Human peripheral blood lymphocytes were stained with biotinylated CD3 (clone UCHT1) (filled histogram) or mouse IgG1 isotype control (open histogram), followed with Sav-Brilliant Violet 570™.
See Brilliant Violet 570™ spectral data
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405227 100 µL 220 CHF
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Description

Streptavidin binds to biotin with high affinity. Streptavidin-Brilliant Violet 570™ is useful for detecting biotinylated antibodies. The excitation of Brilliant Violet 570™ by 405 nm laser light induces a fluorescence maximum emission of 570 nm.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
Streptavidin was conjugated with Brilliant Violet 570™ under optimal conditions.
Concentration
0.1 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The streptavidin solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

ICFC - Verified
Recommended Usage

Each lot of this Streptavidin-Brilliant Violet 570™ is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the Brilliant Violet 570™ conjugation. For immunofluorescent staining, we recommend using ≤0.25 µg in 100 µl staining volume per million cells. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 570™ excites at 405 nm and emits at 570 nm. The bandpass filter 585/42 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 570™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The average molecular weight of Streptavidin-Brilliant Violet 570™ is 340 kD and Streptavidin alone is 52 kD.

Application References

(PubMed link indicates BioLegend citation)
  1. Marandu TF, et al. 2014. Exp Gerontol. 54:101. PubMed
  2. Neumann B, et al. 2015. J Leukoc Biol. 97:19. PubMed
Product Citations
  1. Hartana CA, et al. 2022. Cell Rep. 40:111126. PubMed
  2. Ye Y, et al. 2022. Nat Commun. 13:6458. PubMed
  3. Marandu T, et al. 2014. Exp Gerontol. 54:101. PubMed
  4. Wei Q, et al. 2020. Dev Cell. 53:503. PubMed
  5. Dekhtiarenko I, et al. 2016. PLoS Pathog. 12:e1006072. PubMed
  6. Neumann B, et al. 2015. J Leukoc Biol. 97:19. PubMed
Go To Top Version: 4    Revision Date: 06.18.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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