- Clone
- SA275A11 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Ms4a1, Ly-44
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![SA275A11_BV605_CD20_Antibody_05102021.png SA275A11_BV605_CD20_Antibody_05102021.png](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/SA275A11_BV605_CD20_Antibody_05102021.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse splenocytes were stained with anti-mouse/human CD45R/B220 APC and anti-mouse CD20 (clone SA275A11) Brilliant Violet™ 605 (left) or rat IgG2b, κ Brilliant Violet™ 605 isotype control (right).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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150435 | 50 µg | 314 CHF |
CD20 is a 33-37 kD protein, a member of the MS4A family, with four transmembrane spanning regions that present as a homo-oligomeric complexes in the cell surface when associating with MHC class I and II, CD53, CD81, and CD82. CD20 is expressed on B cells and a subset of T cells, but not on plasma cells. CD20 regulates B-cell activation and proliferation. Its ligation promotes transmembrane Ca2+ trafficking. CD20 is an important therapeutic target in the treatment of B cell lymphomas and leukemias.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse CD20 - transfected cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
- RRID
-
AB_2892307 (BioLegend Cat. No. 150435)
Antigen Details
- Structure
- Member of the MS4A family, four transmembrane spanning regions, three isoforms of 37, 35, and 37 kD, depending on the phosphorylation degree.
- Distribution
-
B cells and a subset of T cells.
- Function
- Regulates B-cell activation and proliferation.
- Interaction
- Associated with MHC class I and II, CD53, CD81, and CD82.
- Cell Type
- B cells, T cells
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Morsy DE, et al. 2013. J. Immunol. 191:3112.
2. Lund FE, Randall TD. 2010. Nat. Rev. Immunol. 10:236.
3. Beers SA, et al. 2010. Blood 115:5191.
4. Kuijpers TW, et al. 2010. J. Clin. Invest. 120:214. - Gene ID
- 12482 View all products for this Gene ID
- UniProt
- View information about CD20 on UniProt.org
Related FAQs
Other Formats
View All CD20 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Alexa Fluor® 647 anti-mouse CD20 | SA275A11 | FC,IHC-F |
Brilliant Violet 421™ anti-mouse CD20 | SA275A11 | FC,IHC-F |
FITC anti-mouse CD20 | SA275A11 | FC |
PE anti-mouse CD20 | SA275A11 | FC |
APC anti-mouse CD20 | SA275A11 | FC |
Biotin anti-mouse CD20 | SA275A11 | FC |
Alexa Fluor® 700 anti-mouse CD20 | SA275A11 | FC |
APC/Cyanine7 anti-mouse CD20 | SA275A11 | FC |
PE/Cyanine7 anti-mouse CD20 | SA275A11 | FC |
PerCP/Cyanine5.5 anti-mouse CD20 | SA275A11 | FC |
TotalSeq™-A0192 anti-mouse CD20 | SA275A11 | PG |
TotalSeq™-B0192 anti-mouse CD20 | SA275A11 | PG |
TotalSeq™-C0192 anti-mouse CD20 | SA275A11 | PG |
KIRAVIA Blue 520™ anti-mouse CD20 Antibody | SA275A11 | FC |
PE/Dazzle™ 594 anti-mouse CD20 Antibody | SA275A11 | FC |
Alexa Fluor® 488 anti-mouse CD20 Antibody | SA275A11 | FC |
Brilliant Violet™ 605 anti-mouse CD20 | SA275A11 | FC |
APC/Fire™ 810 anti-mouse CD20 | SA275A11 | FC |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Alexa Fluor® 647 anti-mouse CD20
C57BL/6 mouse splenocytes were stained with IgD FITC and CD2... C57BL/6 mouse frozen spleen section was fixed with 4% parafo... -
Brilliant Violet 421™ anti-mouse CD20
C57BL/6 mouse splenocytes were stained with CD19 APC and CD2... C57BL/6 mouse frozen spleen section was fixed with 4% parafo... -
FITC anti-mouse CD20
C57BL/6 mouse splenocytes were stained with CD45R/B220 APC a... -
PE anti-mouse CD20
C57BL/6 mouse splenocytes were stained with CD45R/B220 APC a... -
APC anti-mouse CD20
C57BL/6 mouse splenocytes were stained with CD45R/B220 PE an... -
Biotin anti-mouse CD20
C57BL/6 mouse splenocytes were stained with CD19 APC and bio... -
Alexa Fluor® 700 anti-mouse CD20
C57BL/6 mouse splenocytes were stained with CD45R/B220 PE an... -
APC/Cyanine7 anti-mouse CD20
C57BL/6 mouse splenocytes were stained with CD45R/B220 FITC ... -
PE/Cyanine7 anti-mouse CD20
C57BL/6 mouse splenocytes were stained with CD45R/B220 FITC ... -
PerCP/Cyanine5.5 anti-mouse CD20
C57BL/6 mouse splenocytes were stained with CD45R/B220 FITC ... -
TotalSeq™-A0192 anti-mouse CD20
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TotalSeq™-B0192 anti-mouse CD20
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TotalSeq™-C0192 anti-mouse CD20
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KIRAVIA Blue 520™ anti-mouse CD20 Antibody
C57BL/6 mouse splenocytes were stained with CD45R/B220 APC a... -
PE/Dazzle™ 594 anti-mouse CD20 Antibody
C57BL/6 mouse splenocytes were stained with CD45R/B220 APC a... -
Alexa Fluor® 488 anti-mouse CD20 Antibody
C57BL/6 mouse splenocytes were stained with CD45R/B220 APC a... -
Brilliant Violet™ 605 anti-mouse CD20
C57BL/6 mouse splenocytes were stained with anti-mouse/human... -
APC/Fire™ 810 anti-mouse CD20
C57BL/6 mouse splenocytes were stained with anti- mouse CD45...
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