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Brilliant Violet 605™ Streptavidin

Pricing & Availability
Regulatory Status
RUO
Other Names
SAv-Brilliant Violet 605™
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Product Citations
publications
SAV_BV605_043012
Human peripheral blood lymphocytes were stained with biotinylated CD3 (filled histogram) or mouse IgG1 isotype control (open histogram), followed with Sav-Brilliant Violet 605™.
  • SAV_BV605_043012
    Human peripheral blood lymphocytes were stained with biotinylated CD3 (filled histogram) or mouse IgG1 isotype control (open histogram), followed with Sav-Brilliant Violet 605™.
See Brilliant Violet 605™ spectral data
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405229 100 µL 231 CHF
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Description

Streptavidin binds to biotin with high affinity. Streptavidin-Brilliant Violet 605™ is useful for detecting biotinylated antibodies. The excitation of Brilliant Violet 605™ by 405 nm laser light induces a fluorescence maximum emission of 603 nm.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
Streptavidin was conjugated with Brilliant Violet 605™ under optimal conditions.
Concentration
0.1 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The streptavidin solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

ICFC - Verified
Recommended Usage

Each lot of this Streptavidin-Brilliant Violet 605™ is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the Brilliant Violet 605™ conjugation. For immunofluorescent staining, we recommend using ≤0.25 µg in 100 µl staining volume per million cells. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The average molecular weight of Streptavidin-Brilliant Violet 605™ is 340 kD and Streptavidin alone is 52 kD.

Application References

(PubMed link indicates BioLegend citation)
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Product Citations
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  28. Arlehamn C, et al. 2014. J Immunol. 193:2931. PubMed
  29. Cartwright E, et al. 2014. J Immunol. 192:4666. PubMed
  30. Sakaguchi S, et al. 2015. J Leukoc Biol. 97:635. PubMed
  31. Lainé A, et al. 2015. J Immunol. 195: 1791-1803. PubMed
  32. Fallet B, et al. 2020. Cell Rep. 30:1013. PubMed
  33. Chen G, et al. 2019. Curr Protoc Cell Biol. 82:e65. PubMed
  34. Ko KH, et al. 2018. Sci Rep. 8:16905. PubMed
  35. Hutter K, et al. 2020. FEBS J. . PubMed
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  37. Schmid M, et al. 2016. PLoS One. 12: 1005676. PubMed
  38. Pineault KM, et al. 2019. Nat Commun. 10:3168. PubMed
  39. Pascual G, et al. 2021. Nature. 599:485. PubMed
  40. Guo H, et al. 2014. J Leukoc Biol. 96:419. PubMed
  41. Delacher M, et al. 2020. Immunity. 52:295. PubMed
  42. Yabas M, et al. 2016. PLoS One. 11: 0146774. PubMed
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  47. Burnett DL, et al. 2021. Immunity. S1074-7613:00457. PubMed
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Go To Top Version: 4    Revision Date: 06.18.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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