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Brilliant Violet 650™ anti-human CD27 Antibody

Pricing & Availability
Clone
O323 (See other available formats)
Regulatory Status
RUO
Workshop
IV T-186
Other Names
S152, T14, TNFRSF7
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
O323_BV650_020312
Human peripheral blood lymphocytes were stained with CD27 (clone O323) Brilliant Violet 650™.
  • O323_BV650_020312
    Human peripheral blood lymphocytes were stained with CD27 (clone O323) Brilliant Violet 650™.
Compare all formats See Brilliant Violet 650™ spectral data
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302827 25 tests 209 CHF
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302828 100 tests 424 CHF
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Description

CD27 is a 50-55 kD type I membrane protein also known as S152 and T14. It is a lymphocyte-specific member of the TNF-receptor superfamily. CD27 is expressed on medullary thymocytes, virtually all mature T cells, some B cells, and NK cells. CD27 binds to CD70 and plays an important role in costimulation of T cell activation, and regulation of B cell differentiation and proliferation. The cytoplasmic domains of CD27 have also been shown to interact with TRAF2 and TRAF5 to elicit NF-κB and SAPK/JNK activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon, Squirrel Monkey
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
Product Citations
  1. Mohiuddin MM, et al. 2022. Xenotransplantation. 29:e12744. PubMed
  2. Ye Y, et al. 2022. Nat Commun. 13:6458. PubMed
  3. Dudley D, et al. 2016. Nat Commun. 7:12204. PubMed
  4. Woodham A, et al. 2016. Papillomavirus Res. 2:21-30. PubMed
  5. Gagne M, et al. 2022. Cell. 185:113. PubMed
  6. Fajgenbaum DC, et al. 2019. J Clin Invest. 130:4451. PubMed
  7. Kuri-Cervantes L, et al. 2020. STAR Protoc. 1:100154. PubMed
  8. Szabo PA, et al. 2021. Immunity. 54(4):797-814.e6. PubMed
  9. Aliota M, et al. 2016. PLoS Negl Trop Dis. 10:e0005168. PubMed
  10. Jegaskanda S, et al. 2019. JCI Insight. 4:e129035. PubMed
  11. Vanshylla K, et al. 2022. Cell Host Microbe. 30:69. PubMed
  12. Ravimohan S, et al. 2016. Clin Infect Dis. 62: 795 - 803. PubMed
  13. Fromentin R, et al. 2019. Nat Commun. 10:814. PubMed
  14. Phad GE, et al. 2022. Nat Immunol. 23:1. PubMed
  15. Japp AS, et al. 2021. Cell. 184(3):827-839.e14. PubMed
  16. Kiniry B, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.100. PubMed
  17. McCracken M, et al. 2017. PLoS Pathog.. 10.1371/journal.ppat.1006487. PubMed
  18. Buggert M, et al. 2020. Cell. 183(7):1946-1961.e15. PubMed
  19. Kiniry BE, et al. 2018. J Immunol. 200:1876. PubMed
  20. Pinto D, et al. 2020. Cell Host & Microbe. 26(5):623-637.e8.. PubMed
  21. McBrien JB, et al. 2020. Nature. 578:154. PubMed
  22. Fromentin R, et al. 2016. PLoS Pathog. 12: 1005761. PubMed
  23. Pombo C, et al. 2015. J Infect Dis. 12: 1376-1386. PubMed
  24. Burel JG, et al. 2019. Elife. 8. PubMed
  25. Pardons M, et al. 2020. Cell Reports. 29(9):2783-2795.e5.. PubMed
RRID
AB_11124941 (BioLegend Cat. No. 302827)
AB_11124941 (BioLegend Cat. No. 302828)

Antigen Details

Structure
TNF-R superfamily, type I transmembrane glycoprotein, 50-55 kD
Distribution

Medullary thymocytes, T and B cell subsets, NK cells

Function
T cell costimulation
Ligand/Receptor
CD70
Cell Type
B cells, NK cells, T cells, Thymocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Hintzen R, et al. 1994. Immunol. Today 15:307.
2. Agematsu K, et al. 1995. J. Immunol. 154:3627.

Gene ID
939 View all products for this Gene ID
UniProt
View information about CD27 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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