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Brilliant Violet 650™ anti-mouse CD197 (CCR7) Antibody

Pricing & Availability
Clone
4B12 (See other available formats)
Regulatory Status
RUO
Other Names
C-C chemokine receptor type 7 (CCR7), MIP-3 beta receptor, EBV-induced G protein coupled receptor 1, EBI-1, CD197
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
4B12_BV650_-CD197_Antibody_061723
C57BL/6 splenocytes were stained with anti-mouse CD3ε FITC and anti-mouse CD197 (CCR7) (clone 4B12) Brilliant Violet 650™ (left) or rat IgG2a, κ Brilliant Violet 650™ isotype control (right).
  • 4B12_BV650_-CD197_Antibody_061723
    C57BL/6 splenocytes were stained with anti-mouse CD3ε FITC and anti-mouse CD197 (CCR7) (clone 4B12) Brilliant Violet 650™ (left) or rat IgG2a, κ Brilliant Violet 650™ isotype control (right).
Compare all formats See Brilliant Violet 650™ spectral data
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120137 50 µg 305 CHF
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Description

CD197 is also known as C-C chemokine receptor 7 (CCR7) or EBI-1. CCR7 is a G-coupled rhodopsin-like member of the GPCR superfamily with a predicted molecular weight of 43 kD that is expressed on hematopoietic stem cells, most naive T cells, some memory T cells, B subset, and mature dendritic cells. CCR7 is a receptor for the chemokines CCL19 (MIP3 beta) and SLC (6CKine, Exodus-2, TCA-4, CCL21) that plays a role in thymocytes development, T cell adhesion at intestinal sites, the homeostatic recirculation of memory T cells, and chemotaxis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CCR7 transfected RBL-2H3 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The 4B12 antibody does not inhibit binding of ligand to receptor. Additional reported applications (for the relevant formats) include: immunoprecipitation. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302) is recommended prior to immunofluorescent staining.

Staining with clone 4B12 is recommended at 37°C (see supplemental data of PE staining at differing temperatures).

Application References

(PubMed link indicates BioLegend citation)
  1. Ohl L, et al. 2004. Immunity 21:279.
  2. Ritter U, et al. 2004. J. Leukocyte Biol. 76:472.
  3. Lan YY, et al. 2005. Am. J. Transplant. 5:2649. (FC)
  4. Lee JH, et al. 2007. J. Immunol. 178:301. (FC) PubMed
  5. Kurooka M and Kaneda Y. 2007. Cancer Res. 67:227. (FC) PubMed
  6. Thompson BD. 2007. J. Biol. Chem. 282:9547. (FC)
  7. Sakai N, et al. 2006. P. Natl. Acad. Sci. USA 103:14098. (FC)
  8. Turnquist HR, et al. 2007. J. Immunol. 178:7018. (FC)
  9. Hwang IY, et al. 2007. J. Immunol. 179:439. (FC) PubMed
  10. Kang SG, et al. 2007. J. Immunol. 179:3724.
  11. Mao A et al. 2005. J. Immunol. 175:5146. PubMed
  12. Allende ML, et al. 2008. FASEB J. 22:307. PubMed
  13. Kang SG, et al. 2007. J. Immunol. 179:3724. PubMed
  14. Chen H, et al. 2005. J. Immunol. 175:591. PubMed
  15. Florido M, et al. 2009. Immunobiology. 214:643. PubMed
  16. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  17. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
RRID
AB_3068094 (BioLegend Cat. No. 120137)

Antigen Details

Structure
Rhodopsin-like GPCR superfamily, G-protein coupled receptor 1 family, integral membrane protein, predicted molecular weight 43 kD
Distribution

Hematopoietic stem cells, T subsets, mature dendritic cells

Function
Homozygous mutation. Receptor for MIP-3 beta and SLC chemokines. Probable mediator of EBV effects on B lymphocytes. Plays a role in T cell adhesion at intestinal sites; may also play a role in the homeostatic recirculation of memory T cells and chemotaxis.
Ligand/Receptor
MIP3 beta, SLC (6CKine, Exodus-2, TCA-4)
Cell Type
Dendritic cells, Hematopoietic stem and progenitors, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Schweickart VL, et al. 1994. Genomics 23:643.
2. Yoshida R, et al. 1998. J. Biol. Chem. 273:7118.
3. Campbell JJ, et al. 1998. J. Cell Biol. 141:1053.
4. Willimann K, et al. 1998. Eur. J. Immunol. 28:2025.

Gene ID
12775 View all products for this Gene ID
UniProt
View information about CD197 on UniProt.org

Related FAQs

Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

Go To Top Version: 1    Revision Date: 06.16.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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