- Clone
- RTK4530 (See other available formats)
- Regulatory Status
- RUO
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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400651 | 50 µg | 270 CHF |
The isotype of RTK4530 immunoglobulin is rat IgG2b, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
Product DetailsProduct Details
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Trinitrophenol + KLH
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
ICFC - Verified
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest.
Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
- Application Notes
-
The RTK4530 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, and immunofluorescence microscopy. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 400643, 400644, 400671, 400672, 400675, and 400676) as negative control.
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Application References
(PubMed link indicates BioLegend citation) -
- Cervantes-Barragan L, et al. 2007. Blood 109:1131.
- Zeiser R, et al. 2007. Blood 109:2225.
- Sasaki K, et al. 2008. J. Immunol. 181:104. PubMed
- Duan J, et al. 2008. P. Natl. Acad. Sci. USA 105:5183. PubMed
- Yi H, et al. 2009. Blood 113:5819. PubMed
- Schafeer JS, et al. 2010. J. Leukocyte Biol. 87:301. PubMed
- Lei GS, et al. 2015. Infect Immun. 83:572. PubMed
- Richards J, et al. 2015. Mol Cell Cardiol. 79:21. PubMed
- Product Citations
-
- RRID
-
AB_3097674 (BioLegend Cat. No. 400651)
Antigen Details
- Gene ID
- NA
Related Pages & Pathways
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Other Formats
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC Rat IgG2b, κ Isotype Ctrl
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Biotin Rat IgG2b, κ Isotype Ctrl
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FITC Rat IgG2b, κ Isotype Ctrl
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PE Rat IgG2b, κ Isotype Ctrl
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PE/Cyanine5 Rat IgG2b, κ Isotype Ctrl
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Purified Rat IgG2b, κ Isotype Ctrl
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PE/Cyanine7 Rat IgG2b, κ Isotype Ctrl
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APC/Cyanine7 Rat IgG2b, κ Isotype Ctrl
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Alexa Fluor® 647 Rat IgG2b, κ Isotype Ctrl
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Alexa Fluor® 488 Rat IgG2b, κ Isotype Ctrl
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Pacific Blue™ Rat IgG2b, κ Isotype Ctrl
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Alexa Fluor® 700 Rat IgG2b, κ Isotype Ctrl
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PerCP Rat IgG2b, κ Isotype Ctrl
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PerCP/Cyanine5.5 Rat IgG2b, κ Isotype Ctrl
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Brilliant Violet 421™ Rat IgG2b, κ Isotype Ctrl
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Brilliant Violet 570™ Rat IgG2b, κ Isotype Ctrl
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Ultra-LEAF™ Purified Rat IgG2b, κ Isotype Ctrl
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Brilliant Violet 510™ Rat IgG2b, κ Isotype Ctrl
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Brilliant Violet 605™ Rat IgG2b, κ Isotype Ctrl
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Brilliant Violet 785™ Rat IgG2b, κ Isotype Ctrl
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Brilliant Violet 650™ Rat IgG2b, κ Isotype Ctrl
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Brilliant Violet 711™ Rat IgG2b, κ Isotype Ctrl
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PE/Dazzle™ 594 Rat IgG2b, κ Isotype Ctrl
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Alexa Fluor® 594 Rat IgG2b, κ Isotype Ctrl
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GoInVivo™ Purified Rat IgG2b, κ Isotype Ctrl
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APC/Fire™ 750 Rat IgG2b, κ Isotype Ctrl
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TotalSeq™-A0095 Rat IgG2b, κ Isotype Ctrl
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TotalSeq™-C0095 Rat IgG2b, κ Isotype Ctrl
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KIRAVIA Blue 520™ Rat IgG2b, κ Isotype Ctrl
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Spark Blue™ 550 Rat IgG2b, κ Isotype Ctrl
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PE/Fire™ 640 Rat IgG2b, κ Isotype Ctrl
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APC/Fire™ 810 Rat IgG2b, κ Isotype Ctrl
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PE/Fire™ 700 Rat IgG2b, κ Isotype Ctrl
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TotalSeq™-B0095 Rat IgG2b, κ Isotype Ctrl
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TotalSeq™-D0095 Rat IgG2b, κ Isotype Ctrl
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PE/Fire™ 810 Rat IgG2b, κ Isotype Ctrl
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Brilliant Violet 750™ Rat IgG2b, κ Isotype Ctrl
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Spark YG™ 570 Rat IgG2b, κ Isotype Ctrl
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Spark NIR™ 685 Rat IgG2b, κ Isotype Ctrl
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Spark Blue™ 574 Rat IgG2b, κ Isotype Ctrl
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PerCP/Fire™ 806 Rat IgG2b, κ Isotype Ctrl
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Spark Blue™ 515 Rat IgG2b, κ Isotype Ctrl
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PerCP/Fire™ 780 Rat IgG2b, κ Isotype Ctrl
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Spark Red™ 718 Rat IgG2b, κ Isotype Ctrl
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TotalSeq™-Bn0095 Rat IgG2b, κ Isotype Ctrl
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Spark Violet™ 538 Rat IgG2b, κ Isotype Ctrl
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Spark UV™ 387 Rat IgG2b, κ Isotype Ctrl
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Spark PLUS UV395™ Rat IgG2b, κ Isotype Ctrl
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PE/Fire™ 744 Rat IgG2b, κ Isotype Ctrl
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