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Brilliant Violet 711™ anti-human GARP (LRRC32) Antibody

Pricing & Availability
Clone
7B11 (See other available formats)
Regulatory Status
RUO
Other Names
Garpin, Glycoprotein A repetitions predominant
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
7B11_BV-711_GARP_Antibody_010424
Human peripheral blood mononuclear cells were stimulated with purified anti-human CD3 (Cat. No. 300331), purified anti-human CD28 (Cat. No. 302933), and recombinant human IL-2 (Cat. No. 791904) for 24 hours. Cells were stained with anti-human CD4 (clone RPA-T4) Brilliant Violet 421™, anti-human LAP (TGF-β1) (clone S20006A) FITC and anti-human GARP (LRRC32) (clone 7B11) Brilliant Violet 711™ (left) or mouse IgG2b, κ Brilliant Violet 711™ isotype control (right). Data was analyzed by gating on CD4 positive cells.
  • 7B11_BV-711_GARP_Antibody_010424
    Human peripheral blood mononuclear cells were stimulated with purified anti-human CD3 (Cat. No. 300331), purified anti-human CD28 (Cat. No. 302933), and recombinant human IL-2 (Cat. No. 791904) for 24 hours. Cells were stained with anti-human CD4 (clone RPA-T4) Brilliant Violet 421™, anti-human LAP (TGF-β1) (clone S20006A) FITC and anti-human GARP (LRRC32) (clone 7B11) Brilliant Violet 711™ (left) or mouse IgG2b, κ Brilliant Violet 711™ isotype control (right). Data was analyzed by gating on CD4 positive cells.
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352521 25 tests 230 CHF
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352522 100 tests 460 CHF
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Description

Glycoprotein A Repetitions Predominant (GARP), also known as leucine rich repeat containing 32 (LRC32), is a 80 kD type I membrane glycoprotein with 20 leucine rich repeats in the extracellular portion of the protein. GARP was found on the surface of megakaryocytes, platelets, and activated Tregs (CD4+, CD25+, FoxP3+ cells) and serves as a receptor for latent TGF-β. Recent evidence suggests that GARP may play a role in controlling suppressor function of Tregs. A mutation in GARP has been reported in a large Samaritan kindred with Usher syndrome type 1, an autosomal recessive disease characterized by profound congenital sensorineural deafness, vestibular dysfunction, and progressive visual loss. In addition, it has been found that GARP mRNA is highly amplified in different tumors, which indicates that tumor cells may use GARP to express TGF-β or to capture TGF-β from their surroundings, resulting in local suppression of anti-tumor immune responses or the induction of Tregs.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
LRRC32-DNA vaccination
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 711™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 711™ excites at 405 nm and emits at 711 nm. The bandpass filter 710/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 711™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
RRID
AB_3097493 (BioLegend Cat. No. 352521)
AB_3097493 (BioLegend Cat. No. 352522)

Antigen Details

Structure
80 kD transmembrane GARP glycoprotein with an extracellular region containing 20 leucine-rich repeats
Distribution

Activated Tregs, megakaryocytes, platelets

Ligand/Receptor
LAP (TGF-β1)
Cell Type
Megakaryocytes, Platelets, Tregs
Biology Area
Cell Biology, Immunology, Signal Transduction
Antigen References

1. Ollendorff V, et al. 1994. Cell. Growth Differ. 5:213.
2. Stockis J, et al. 2009. Eur. J. Immunol. 39:3315.
3. Wang R, et al. 2009. P. Natl. Acad. Sci. USA 106:13439.
4. Tran DQ, et al. 2009. P. Natl. Acad. Sci. USA 106:13445.

Gene ID
2615 View all products for this Gene ID
UniProt
View information about GARP on UniProt.org
Go To Top Version: 1    Revision Date: 01.04.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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