- Clone
- BJ18 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- VI A034
- Other Names
- Hermes, Pgp-1, H-CAM, HUTCH-1, ECMR III, gp85, Ly-24
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![BJ18_BV785_CD44_Antibody_042920 BJ18_BV785_CD44_Antibody_042920](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/BJ18_BV785_CD44_Antibody_042920.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood lymphocytes were stained with CD44 (clone BJ18) Brilliant Violet 785™ (filled histogram) or mouse IgG1, κ Brilliant Violet 785™ isotype control (open histogram).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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338833 | 25 tests | 209 CHF | ||||
338834 | 100 tests | 407 CHF |
CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from a low or mid level of intensity to high expression levels. Thus, CD44 has been reported to be a valuable marker for memory cell subsets. CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon, Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Normal human PBL
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 785™ under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 785™ excites at 405 nm and emits at 785 nm. The bandpass filter 780/60 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 785™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
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Violet Laser (405 nm)
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Application References
(PubMed link indicates BioLegend citation) -
- Kishimoto T, et al. eds. 1997 Leucocyte Typing VI:White Cell Differentiation Antigen. Garland Publishing Inc.
- RRID
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AB_2860873 (BioLegend Cat. No. 338833)
AB_2860874 (BioLegend Cat. No. 338834)
Antigen Details
- Structure
- Variable splicing of CD44 gene generates many CD44 isoforms, 85 kD
- Distribution
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All leukocytes, epithelial cells, endothelial cells, hepatocytes, mesenchymal cells
- Function
- Leukocyte attachment and rolling on endothelial cells, stromal cells and ECM
- Ligand/Receptor
- Hyaluronan, MIP-1β, fibronectin, collagen
- Cell Type
- Endothelial cells, Epithelial cells, Leukocytes, Mesenchymal cells, Mesenchymal Stem Cells, Tregs
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Stem Cells
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
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1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Haynes BF, et al. 1991. Cancer Cells 3:347.
3. Goldstein LA, et al. 1989. Cell 56:1063.
4. Mikecz K, et al. 1995. Nat. Med. 1:558. - Gene ID
- 960 View all products for this Gene ID
- UniProt
- View information about CD44 on UniProt.org
Related FAQs
Other Formats
View All CD44 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human CD44 | BJ18 | FC,CyTOF®,ICC |
FITC anti-human CD44 | BJ18 | FC |
APC anti-human CD44 | BJ18 | FC |
PE anti-human CD44 | BJ18 | FC |
Brilliant Violet 421™ anti-human CD44 | BJ18 | FC |
Purified anti-human CD44 (Maxpar® Ready) | BJ18 | FC,CyTOF® |
Alexa Fluor® 700 anti-human CD44 | BJ18 | FC |
PE/Dazzle™ 594 anti-human CD44 | BJ18 | FC |
PE/Cyanine7 anti-human CD44 | BJ18 | FC |
APC/Fire™ 750 anti-human CD44 | BJ18 | FC |
PerCP/Cyanine5.5 anti-human CD44 | BJ18 | FC |
Pacific Blue™ anti-human CD44 | BJ18 | FC |
TotalSeq™-A0125 anti-human CD44 | BJ18 | PG |
TotalSeq™-C0125 anti-human CD44 | BJ18 | PG |
Alexa Fluor® 488 anti-human CD44 | BJ18 | FC |
Brilliant Violet 785™ anti-human CD44 | BJ18 | FC |
TotalSeq™-B0125 anti-human CD44 Antibody | BJ18 | PG |
TotalSeq™-D0125 anti-human CD44 | BJ18 | PG |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD44
MDA-MB-231 cells were stained with anti-CD44 (clone BJ18), f... -
FITC anti-human CD44
Human peripheral blood lymphocytes stained with BJ18 FITC -
APC anti-human CD44
Human peripheral blood lymphocytes stained with BJ18 APC -
PE anti-human CD44
Human peripheral blood lymphocytes stained with BJ18 PE Pre-lysed human blood leukocytes were stained with CD44 (clo... -
Brilliant Violet 421™ anti-human CD44
Human peripheral blood lymphocytes were stained with CD44 (c... -
Purified anti-human CD44 (Maxpar® Ready)
Human PBMCs stained with 166Er-anti-CD44 (BJ18) and 158Gd-an... -
Alexa Fluor® 700 anti-human CD44
Human peripheral blood lymphocytes were stained with CD44 (c... -
PE/Dazzle™ 594 anti-human CD44
Human peripheral blood lymphocytes were stained with CD44 (c... -
PE/Cyanine7 anti-human CD44
Human peripheral blood lymphocytes were stained with CD44 (c... -
APC/Fire™ 750 anti-human CD44
Human peripheral blood lymphocytes were stained with CD44 (c... -
PerCP/Cyanine5.5 anti-human CD44
Human peripheral blood lymphocytes were stained with CD44 (c... -
Pacific Blue™ anti-human CD44
Human peripheral blood lymphocytes were stained with CD44 (c... -
TotalSeq™-A0125 anti-human CD44
-
TotalSeq™-C0125 anti-human CD44
-
Alexa Fluor® 488 anti-human CD44
Human peripheral blood lymphocytes were stained with CD3 APC... -
Brilliant Violet 785™ anti-human CD44
Human peripheral blood lymphocytes were stained with CD44 (c... -
TotalSeq™-B0125 anti-human CD44 Antibody
-
TotalSeq™-D0125 anti-human CD44
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