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- Clone
- A15153G (See other available formats)
- Regulatory Status
- RUO
- Other Names
- T-cell immunoreceptor with Ig and ITIM domains, VSIG9, VSTM3, WUCAM
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Human peripheral blood lymphocytes were stained with CD3 PE and TIGIT (VSTM3) (clone A15153G) Brilliant Violet 785™ (left) or mouse IgG2a, κ Brilliant Violet 785™ isotype control (right).
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 372735 | 25 tests | 226 CHF | ||||
| 372736 | 100 tests | 457 CHF | ||||
T cell immunoreceptor with Ig and ITIM domains (TIGIT), also known as VSTM3 or WUCAM, is a 26 kD, type I transmembrane protein and is a member of the PVR (poliovirus receptor) family of immunoglobulin-like domain containing proteins. TIGIT is expressed on activated T cells, follicular T helper, memory, and regulatory T cells as well as on NK cells. TIGIT is a negative regulator of NK and T cell activation. Expression of TIGIT is associated with decreased functionality of CD8 T cells in chronic viral infection and tumors. TIGIT also promotes the differentiation of tolerogenic phenotype in dendritic cells with an increased secretion of IL-10 and a diminished production of IL-12.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant Human TIGIT.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 785™ under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 785™ excites at 405 nm and emits at 785 nm. The bandpass filter 780/60 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 785™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
- Application Notes
-
This clone can suppress anti-CD3 induced T cell proliferation in vitro based on in-house testing.
This clone has been tested in-house and determined to not be suitable for applications in immunohistochemistry of paraffin-embedded tissue sections (IHC-P).
Additional reported applications (for the relevant formats) include: Blocking1. -
Application References
(PubMed link indicates BioLegend citation) -
- Stamm H, et al. 2018. Oncogene. Pubmed
- RRID
-
AB_2892455 (BioLegend Cat. No. 372735)
AB_2892455 (BioLegend Cat. No. 372736)
Antigen Details
- Structure
- 26kD; type I transmembrane protein, Ig-like V-type domain, ITIM motif.
- Distribution
-
Activated T cells, Regulatory T cells (Treg), Follicular Helper T cells (TFH), NK cells.
- Function
- Cell signaling, negative regulation of T cells, T cell tolerance, T cell anergy.
- Ligand/Receptor
- CD155 (PVR), CD112 (PVRL2, NECTIN-2).
- Cell Type
- NK cells, T cells, Tfh, Tregs
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Inhibitory Molecules, Signal Transduction
- Molecular Family
- Adhesion Molecules, Immune Checkpoint Receptors
- Antigen References
-
1. Stanietsky N, et al. 2009. Proc. Natl. Acad. Sci. 106:17858.
2. Yu X, et al. 2009. Nat. Immunol. 10:48.
3. Johnston R, et al. 2014. Cancer Cell. 26:923. - Gene ID
- 201633 View all products for this Gene ID
- UniProt
- View information about TIGIT on UniProt.org
Related FAQs
Other Formats
View All TIGIT (VSTM3) Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human TIGIT (VSTM3)
Human peripheral blood leukocytes were stained with CD3 FITC... 
Human PBMCs were labeled with CFSE. Cells were cultured in ... -
APC/Fire™ 750 anti-human TIGIT (VSTM3)
Human peripheral blood leukocytes were stained with CD3 FITC... 
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APC anti-human TIGIT (VSTM3)
Human peripheral blood leukocytes were stained with CD3 FITC... 
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PE anti-human TIGIT (VSTM3)
Human peripheral blood leukocytes were stained with CD3 APC ... 
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Brilliant Violet 421™ anti-human TIGIT (VSTM3)
Human peripheral blood leukocytes were stained with CD3 FITC... 
-
Brilliant Violet 605™ anti-human TIGIT (VSTM3)
Human peripheral blood leukocytes were stained with CD3 FITC... 
-
PE/Dazzle™ 594 anti-human TIGIT (VSTM3)
Human peripheral blood leukocytes were stained with CD3 FITC... 
-
PerCP/Cyanine5.5 anti-human TIGIT (VSTM3)
Human peripheral blood leukocytes were stained with CD3 APC ... -
PE/Cyanine7 anti-human TIGIT (VSTM3)
Human peripheral blood leukocytes were stained with FITC CD3... -
Ultra-LEAF™ Purified anti-human TIGIT (VSTM3)
Human peripheral blood leukocytes were stained with CD3 FITC... 
Human PBMCs were labeled with CFSE. Cells were cultured in ... -
Biotin anti-human TIGIT (VSTM3)
Human peripheral blood leukocytes were stained with CD3 FITC... 
-
Alexa Fluor® 647 anti-human TIGIT (VSTM3)
Human peripheral blood lymphocytes were stained with CD3 PE ... 
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TotalSeq™-A0089 anti-human TIGIT (VSTM3)
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TotalSeq™-B0089 anti-human TIGIT (VSTM3)
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TotalSeq™-C0089 anti-human TIGIT (VSTM3)
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KIRAVIA Blue 520™ anti-human TIGIT (VSTM3)
Human peripheral blood lymphocytes were stained with CD3 APC... -
APC/Cyanine7 anti-human TIGIT (VSTM3)
Human peripheral blood lymphocytes were stained with CD3 PE ... -
Brilliant Violet 510™ anti-human TIGIT (VSTM3)
Human peripheral blood lymphocytes were stained with CD3 APC... -
Brilliant Violet 785™ anti-human TIGIT (VSTM3) Antibody
Human peripheral blood lymphocytes were stained with CD3 PE ... -
TotalSeq™-D0089 anti-human TIGIT (VSTM3)
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Brilliant Violet 711™ anti-human TIGIT (VSTM3)
Human peripheral blood lymphocytes were stained with CD3 Ale... -
PE/Fire™ 640 anti-human TIGIT (VSTM3)
Human peripheral blood leukocytes were stained with anti-hum... -
PE/Fire™ 810 anti-human TIGIT (VSTM3)
Human peripheral blood lymphocyte were stained with anti-hum... -
PE/Cyanine5 anti-human TIGIT (VSTM3)
Human peripheral blood leukocytes were stained with anti-hum... -
PerCP/Fire™ 806 anti-human TIGIT (VSTM3)
Human peripheral blood lymphocytes were stained with anti-hu... -
PerCP/Fire™ 780 anti-human TIGIT (VSTM3)
Human lysed whole blood cells were stained with anti-human C... -
Spark Red™ 718 anti-human TIGIT (VSTM3) (Flexi-Fluor™)
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PE/Fire™ 700 anti-human TIGIT (VSTM3)
Human peripheral blood lymphocytes were stained with anti-hu...
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