- Clone
- 11-26c.2a (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Immunoglobulin D
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![1126c2a_BV785_IgD_Antibody_061523 1126c2a_BV785_IgD_Antibody_061523](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/1126c2a_BV785_IgD_Antibody_061523.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
-
C57BL/6 mouse splenocytes were stained with anti-mouse B220 PE and anti-mouse IgD (clone 11-26c.2a) Brilliant Violet 785™ (left) or rat IgG2a, κ Brilliant Violet 785™ isotype control (right).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
405757 | 50 µg | 270 CHF |
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 785™ under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 785™ excites at 405 nm and emits at 785 nm. The bandpass filter 780/60 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 785™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
- Application Notes
-
The 11-26c.2a antibody reacts with immunoglobulin D in all tested mouse haplotypes. The antibody binds membrane IgD expressed on most B cells. The 11-26c.2a antibody neither induces proliferation of splenic B cells nor induces B cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections2,3, and spatial biology (IBEX)10,11.
-
Application References
(PubMed link indicates BioLegend citation) -
- Nitschke L, et al. 1993. P. Natl. Acad. Sci. USA 90:1887. (FC)
- Weih D, et al. 2001. J. Immunol. 167:1909. (IHC)
- Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC)
- Ahuja A, et al. 2007. J. Immunol. 179:3351. (FC) PubMed
- Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
- Good-Jacobson KL, et al. 2010. Nat. Immunol. 11:535. (FC) PubMed
- Tomayko MM, et al. 2010. J. Immunol. 185:7146. PubMed
- Park SY, et al. 2013. J. Immunol. 190:1094. PubMed
- Rouaud P, et al. 2014. J Exp Med. 211:975. PubMed
- Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
- RRID
-
AB_3068111 (BioLegend Cat. No. 405757)
Antigen Details
- Structure
- Ig family
- Distribution
-
B cells
- Function
- B cell differentiation
- Cell Type
- B cells
- Biology Area
- Immunology
- Gene ID
- 380797 View all products for this Gene ID
- UniProt
- View information about IgD on UniProt.org
Related FAQs
Other Formats
View All IgD Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
FITC anti-mouse IgD
C57BL/6 mouse splenocytes stained with 11-26c.2a FITC -
PE anti-mouse IgD
C57BL/6 splenocytes stained with 11-26c.2a PE -
Purified anti-mouse IgD
C57BL/6 mouse splenocytes stained with purified 11-26c.2a, f... -
PerCP anti-mouse IgD
C57BL/6 mouse splenocytes were stained with CD45R/B220 APC a... -
Biotin anti-mouse IgD
C57BL/6 splenocytes were stained with CD45R/B220 APC and bio... -
Brilliant Violet 711™ anti-mouse IgD
C57BL/6 mouse splenocytes were stained with B220 APC and IgD... -
Alexa Fluor® 700 anti-mouse IgD
C57BL/6 mouse splenocytes were stained with B220 FITC and Ig... Confocal image of C57BL/6 mouse spleen sample acquired using... Confocal image of C57BL/6 mouse small intestine sample acqui... -
Alexa Fluor® 647 anti-mouse IgD
C57BL/6 splenocytes stained with 11-26c.2a Alexa Fluor® 647 Paraformaldehyde-fixed (4%), 500 μm-thick mouse spleen secti... -
PerCP/Cyanine5.5 anti-mouse IgD
C57BL/6 splenocytes stained with 11-26c.2a PerCP/Cyanine5.5 -
Pacific Blue™ anti-mouse IgD
C57BL/6 splenocytes stained with 11-26c.2a Pacific Blue&trad... C57BL/6 splenocytes stained with rat IgG2a Pacific Blue&trad... -
APC anti-mouse IgD
C57BL/6 splenocytes stained with 11-26c.2a APC -
APC/Cyanine7 anti-mouse IgD
C57BL/6 splenocytes stained with 11-26c.2a APC/Cyanine7 -
Alexa Fluor® 488 anti-mouse IgD
C57BL/6 splenocytes were stained with B220 APC and IgD (11-2... Mice were injected subcutaneously with sheep red blood cells... -
PE/Cyanine7 anti-mouse IgD
C57BL/6 splenocytes were stained with CD45R/B220 FITC and Ig... -
Brilliant Violet 650™ anti-mouse IgD
C57BL/6 splenocytes were stained with CD45R/B220 APC and IgD... -
Brilliant Violet 510™ anti-mouse IgD
C57BL/6 splenocytes were stained with CD45R/B220 APC and IgD... -
Brilliant Violet 421™ anti-mouse IgD
C57BL/6 splenocytes were stained with CD45R/B220 APC and IgD... -
Brilliant Violet 605™ anti-mouse IgD
C57BL/6 splenocytes were stained with CD45R/B220 APC and IgD... -
Purified anti-mouse IgD (Maxpar® Ready)
C57BL/6 mouse splenocytes stained with 176Yb-anti-CD45R/B220... -
Alexa Fluor® 594 anti-mouse IgD
C57BL/6 mouse frozen spleen section was fixed with 4% parafo... Paraformaldehyde-fixed (4%), 500 μm-thick mouse spleen secti... -
PE/Dazzle™ 594 anti-mouse IgD
C57BL/6 mouse splenocytes were stained with B220 APC and IgD... -
APC/Fire™ 750 anti-mouse IgD
C57BL/6 mouse splenocytes were stained with CD45R/B220 FITC ... -
TotalSeq™-A0571 anti-mouse IgD
-
TotalSeq™-C0571 anti-mouse IgD
-
Spark NIR™ 685 anti-mouse IgD
C57BL/6 splenocytes were stained with anti-mouse CD45R/B220 ... -
TotalSeq™-B0571 anti-mouse IgD Antibody
-
Spark Violet™ 423 anti-mouse IgD
C57BL/6 splenocytes were stained with anti-mouse/human CD45R... -
PE/Cyanine5 anti-mouse IgD
C57BL/6 mouse splenocytes were stained with anti-mouse B220 ... -
Brilliant Violet 785™ anti-mouse IgD
C57BL/6 mouse splenocytes were stained with anti-mouse B220 ... -
Spark PLUS UV™ 395 anti-mouse IgD
C57BL/6 mouse splenocytes were stained with anti-mouse CD45R...
Follow Us