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c-Myc Peptide Tag (Previously Covance catalog# PEP-150P)

Pricing & Availability
Regulatory Status
RUO
Other Names
(CEQKLISEEDL), Myc Proto-Oncogene Protein, Proto-Oncogene C-Myc, Transcription Factor P64, Class E Basic Helix-Loop-Helix Protein 39, Avian Myelocytomatosis Viral Oncogene Homolog, V-Myc, Myc-Related Translation/Localization
Previously
Covance Catalog# PEP-150P
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Product Citations
publications
Cat # Size Price Quantity Check Availability Save
931701 1 mg 213 CHF
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931702 5 mg 433 CHF
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Description

The c-myc tag is often used as a protein modification in order to simplify the labeling and detection of proteins. Antibodies against c-myc epitopes recognize overexpressed proteins containing Myc epitope tag fused to targeted proteins and allow for specific detection in western blotting, immunoprecipitation, and immunostaining techniques.

Product Details
Technical Data Sheet (pdf)

Product Details

Purity
>90% pure by HPLC
Formulation
Lyophilized.
Storage & Handling
Store lyophilized or at -20°C in solution
Application

IP, WB
Recommended Usage

Soluble in PBS. The optimal working dilution should be determined for each specific assay condition.
• Elution of epitope tag fusion proteins from immunoaffinity columns: 400 ug/mL
• Competitive inhibition of antibody in western or immunostaining: 10-fold molar excess over optimized (low) antibody

Application Notes

This synthetic peptide (H2N-CEQKLISEEDL-COOH) corresponds to the epitope detected by the 9E10 monoclonal antibody.

This peptide can be used in competition experiments, or to elute bound c-myc fusion proteins from the 9E10 affinity matrix.
 

Application References

(PubMed link indicates BioLegend citation)
  1. Enquist-Newman M, et al. 2001. Mol. Biol. Cell. 12: 2601.
  2. Ferrando A, et al. 2001. Nucleic Acids Res. 29:3685.
Go To Top Version: 2    Revision Date: 07.27.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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