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Ce3D™ Tissue Clearing Kit

Pricing & Availability
Regulatory Status
RUO
Other Names
Clearing-enhanced 3D
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Product Citations
publications
Ce3D_Tissue_Clearing_Kit_1_110320.png
Paraformaldehyde-fixed (4%), 500 µm-thick mouse spleen section was processed according to the Ce3D™ Tissue Clearing Kit protocol (Cat. No. 480401). The section was anti-mouse CD172a (SIRPα) (clone P84) Alexa Fluor® 594 at 5 µg/mL (cyan), and anti-mouse CD200 (OX2) (clone OX-90) Alexa Fluor® 647 at 5 µlg/mL (magenta). The section was then optically cleared and mounted in a sample chamber. The image was captured with a 10X objective using Zeiss 780 confocal microscope and processed by Imaris image analysis software. Watch the video.
  • Ce3D_Tissue_Clearing_Kit_1_110320.png
    Paraformaldehyde-fixed (4%), 500 µm-thick mouse spleen section was processed according to the Ce3D™ Tissue Clearing Kit protocol (Cat. No. 480401). The section was anti-mouse CD172a (SIRPα) (clone P84) Alexa Fluor® 594 at 5 µg/mL (cyan), and anti-mouse CD200 (OX2) (clone OX-90) Alexa Fluor® 647 at 5 µlg/mL (magenta). The section was then optically cleared and mounted in a sample chamber. The image was captured with a 10X objective using Zeiss 780 confocal microscope and processed by Imaris image analysis software. Watch the video.
  • Ce3D_Tissue_Clearing_Kit_2_110320.png
    Paraformaldehyde-fixed (4%), 500 µm-thick mouse spleen section was processed according to the Ce3D™ Tissue Clearing Kit protocol (Cat. No. 480401). The section was costained with anti-mouse CD169 (Siglec-1) (clone 3D6.112) Alexa Fluor® 488 at 5 µg/mL (green), anti-mouse I-A/I-E (clone M5/114.15.2) Alexa Fluor® 594 at 5 µg/mL (blue), and anti-mouse CD3ε (clone KT3.1.1) Alexa Fluor® 647 at 5 µg/mL (magenta). The section was then optically cleared and mounted in a sample chamber. The image was captured with a 10X objective using Zeiss 780 confocal microscope and processed by Imaris image analysis software. Watch the video.
  • Ce3D_Tissue_Clearing_Kit_3_110320.png
    Paraformaldehyde-fixed (4%), mouse intestine section was processed according to the Ce3D™ Tissue Clearing Kit protocol (Cat. No. 480401). The section was costained with anti-Tubulin β 3 (TUBB3) (clone TUJ1) Alexa Fluor® 594 at 5 µg/mL (green), and anti-mouse CD45 (clone 30-F11) Alexa Fluor® 647 at 5 µg/mL (magenta). The section was then optically cleared and mounted in a sample chamber. The image was captured with a 20X objective using Zeiss 780 confocal microscope and processed by Imaris image analysis software. Watch the video.
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427701 10 tests 305 CHF
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427702 50 tests 774 CHF
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Description

Ce3D™ (clearing-enhanced 3D) Tissue Clearing Solution is a hydrophilic, refractive index (RI) matching reagent that is easy-to-use, and allows rapid and robust clearing of diverse tissues. Ce3D™ has an RI of ~1.5 and is compatible with multicolor fluorescence microscopy. The Ce3D™ tissue clearing technique works by simple immersion of tissue into the clearing reagent, and does not require special equipment or containers. Ce3D™ Tissue Clearing Kit includes  Ce3D™ Tissue Clearing Solution, Ce3D™ Permeabilization/Blocking Buffer, Ce3D™ Antibody Diluent Buffer and  Ce3D™ Wash Buffer, which are formulated for use with 3D IHC application.

Product Details
Technical Data Sheet (pdf)

Product Details

Storage & Handling
The buffer solution should be stored between 2°C and 8°C.
Application

IHC-F - Quality tested
3D IHC - Verified
Recommended Usage

Ce3D™ tissue clearing solution is used at 500 µL per test size  (500 µm thick tissue) to cover the tissue.

Ce3D™ Permeabilization/blocking buffer  is used at 500 µL per test size  (500 µm thick tissue) to cover the tissue.

Ce3D™ antibody diluent buffer  is used at 500 µL per test size  (500 µm thick tissue) to cover the tissue.

Ce3D™ wash buffer is used at 500 µL per test size  (500 µm thick tissue) to cover the tissue.

Product Citations
  1. Hsiao FT, et al. 2023. Nat Commun. 14:3395. PubMed
  2. Bolkhovitina EL, et al. 2022. Int J Mol Sci. 23: . PubMed

Antigen Details

Gene ID
NA
Go To Top Version: 1    Revision Date: 11.03.2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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