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Cell-Vive™ CD Blood 55-5 Cryopreservation Solution, GMP

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Other Names
Cryo Solution, cropreservation media
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CV_GMP_RECOM_CD_Blood55-5_Cryo_071124
Human PBMCs were isolated and frozen in 1 mL of either BioLegend Cell-Vive™ CD Blood 55-5 Cryopreservation Solution, GMP (cat#: 420518, black bar) or a Competitor’s cryopreservation solution (white bar) for a minimum of 3 days. Cells were thawed, washed, and resuspended in PBS prior to calculating viability. Cells were thawed at a density of 5 to 15 million cells per mL. The black bar represents replicates from 5 independent lots of Cell-Vive™ CD Blood 55-5 Cryopreservation Solution, GMP.
  • CV_GMP_RECOM_CD_Blood55-5_Cryo_071124
    Human PBMCs were isolated and frozen in 1 mL of either BioLegend Cell-Vive™ CD Blood 55-5 Cryopreservation Solution, GMP (cat#: 420518, black bar) or a Competitor’s cryopreservation solution (white bar) for a minimum of 3 days. Cells were thawed, washed, and resuspended in PBS prior to calculating viability. Cells were thawed at a density of 5 to 15 million cells per mL. The black bar represents replicates from 5 independent lots of Cell-Vive™ CD Blood 55-5 Cryopreservation Solution, GMP.
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420518 50 mL 195 CHF
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Description

Cell-Vive™ CD Blood 55-5 Cryopreservation Solution, GMP is designed to support the cryopreservation of human stem cells, cells isolated from umbilical cord blood, and PBMCs. Cell-Vive™ CD Blood 55-5 Cryopreservation Solution, GMP is a chemically-defined formulation prepared without human or other animal-derived components. It is suitable for use in the cryopreservation of different human primary blood-derived cell sources, without affecting relevant properties. When used under appropriate conditions, this product can preserve cell viability, expansion, and phenotype of these cell types. This GMP product is suggested for use in research and ex vivo cell processing. The benefits of this Cryopreservation solution include:

  • Chemically-defined formulation.
  • Manufactured in a GMP facility according to USP <1043>.
  • Able to maintain important stem cell properties after cryopreservation.

 

 

 

Product Details
Quality Statement

BioLegend Cell-Vive™ GMP cell culture products are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12 in a dedicated GMP facility compliant with ISO 13485:2016. Specifications and processes include

  • Low endotoxin level (< 1 EU/mL)
  • Mycoplasma and bacterial/fungal growth testing
  • Batch-to-batch consistency
  • Vendor qualification
  • Raw material traceability and documentation
  • Documented procedures and employee training
  • Equipment maintenance and monitoring records
  • Lot-specific certificates of analysis
  • QA review of released products
  • Quality audits per ISO 13485:2016
Technical Data Sheet (pdf)

Product Details

Formulation
Chemically-defined. Cell-Vive™ CD Blood 55-5 Cryopreservation Solution, GMP is a solution ready to use.
Endotoxin Level
< 1EU/mL
Preparation
Chemically-Defined, no preservatives.
Storage & Handling
4°C (2-8 °C)
Application

Cell Culture
Recommended Usage

Freeze 0.5-5 million cells per 1mL of Cell-Vive™ CD Blood 55-5 Cryopreservation Solution, GMP

Application Notes

For cryopreservation, a range between 0.5-5 million cells per 1 mL of Cell-Vive™ CD Blood 55-5 Cryopreservation Solution, GMP is recommended. The liquid product is clear in appearance.

 

Cryopreservation protocol:

  1. Prepare a cell suspension following an appropriate protocol.
  2. Centrifuge cells at 300 x g for 5 minutes to obtain a pellet. Carefully aspirate supernatant
  3. Resuspend pelleted cells with Cell-Vive™ CD Blood 55-5 Cryopreservation Solution, GMP and dispense the cell suspension into a cryovial.
  4. Immediately transfer the cryovial into a cell freezing container and store it at -80°C.
  5. After 24-36 hours, transfer cryovials into a liquid nitrogen tank for long-term storage.

Thawing protocol

  1. Thaw cryovials containing cells by gently swirling them in a 37°C water bath. Thaw until only a small ice fragment is present.
  2. In a biosafety hood, add the thawed cryopreservation solution into 8 mL of preferred cell culture media in a 15 mL conical tube. Wash the vial with 1 mL of cell culture media and add to the conical tube containing cells. Gently mix.
  3. Centrifuge and remove the supernatant.

Resuspend the cells at the desired density with cell culture media.

Disclaimer

BioLegend Cell-Vive™ GMP Cell Culture products are for research use only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

Antigen Details

Gene ID
NA
Go To Top Version: 1    Revision Date: 08.28.2024

For Research Use Only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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