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Cell-Vive™ GMP Ultra-LEAF™ Purified anti-HU CD69 RecAb SF

Pricing & Availability
Clone
QA18A26 (See other available formats)
Other Names
Very Early Activation Antigen (VEA), Activation inducer molecule (AIM)
Isotype
Mouse IgG1, κ
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Product Citations
publications
QA18A26_CV_GMP_ULEAF_CD69_RecAb_SF_053124
Human peripheral blood lymphocytes stimulated with BioLegend Cell Activation Cocktail (cat#: 423302) for 2 days were stained with Cell-Vive™ GMP Ultra-LEAF™ anti-human CD69 Purified RecAb SF at 0.5 µg per test (Clone QA18A26, cat#: 385703, filled histogram) or an isotype control (Clone MOPC-21, cat#: 400101, open histogram). Both primary stains were followed by an anti-mouse IgG FITC secondary stain (Clone Poly4060, cat#: 406001).
  • QA18A26_CV_GMP_ULEAF_CD69_RecAb_SF_053124
    Human peripheral blood lymphocytes stimulated with BioLegend Cell Activation Cocktail (cat#: 423302) for 2 days were stained with Cell-Vive™ GMP Ultra-LEAF™ anti-human CD69 Purified RecAb SF at 0.5 µg per test (Clone QA18A26, cat#: 385703, filled histogram) or an isotype control (Clone MOPC-21, cat#: 400101, open histogram). Both primary stains were followed by an anti-mouse IgG FITC secondary stain (Clone Poly4060, cat#: 406001).
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385704 1 mg 1683 CHF
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385703 100 µg 328 CHF
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Description

CD69, also known as activation inducer molecule (AIM), very early activation antigen (VEA), and MLR3, is a 27-33 kD type II transmembrane protein. It is a member of the C-type lectin family and expressed as a disulfide-linked homodimer. Other members of this receptor family include NKG2, NKR-P1 CD94, and Ly49. CD69 is transiently expressed on activated leukocytes including T cells, thymocytes, B cells, NK cells, neutrophils, and eosinophils. CD69 is constitutively expressed by a subset of medullary mature thymocytes, platelets, mantle B cells, and certain CD4+ T cells in germinal centers of normal lymph nodes. CD69 is involved in early events of lymphocyte, monocyte, and platelet activation, and has a functional role in redirected lysis mediated by activated NK cells.membrane and is tested negative for mycoplasma and microbial growth. Cell-Vive™ GMP Ultra-LEAF™ Biotin anti-human CD34 SF Antibody is for research and further ex vivo cell processing use only.

Product Details
Quality Statement

BioLegend Cell-Vive™ GMP Ultra-LEAF™ antibodies are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12 in a dedicated GMP facility compliant with ISO 13485:2016. Specifications and processes include

  • Ultra-Low endotoxin level (<0.01 EU/µg)
  • Manufactured under serum-free conditions
  • Bulks tested negative for mycoplasma and bacterial/fungal growth
  • Serum-free hybridomas are negative for the presence of murine virus
  • Batch-to-batch consistency
  • Vendor qualification
  • Raw material traceability and documentation
  • Documented procedures and employee training
  • Equipment maintenance and monitoring records
  • Lot-specific certificates of analysis
  • QA review of released products
  • Quality audits per ISO 13485:2016
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Recombinant
Host Species
Mouse
Formulation
0.1 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Endotoxin Level
Less than 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test
Concentration
1.0 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone QA18A26 can completely block the binding of clone FN50 on target cells.

 

This GMP Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.1 µm filtered) is recommended for highly sensitive assays.

Disclaimer

BioLegend Cell-Vive™ GMP Ultra-LEAF™ antibodies are for research use only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

Antigen Details

Structure
C-type lectin, type II glycoprotein, 28/32 kD
Distribution

Activated T cells, B cells, NK cels, Granulocytes, thymocytes, platelets, Langerhans cells

Function
Lymphocyte, monocyte, and platelet activation, NK cell killing
Ligand/Receptor
Galectin-1, S100A8/S100A9 complex, myosin light chains 9 and 12, and oxidized low-density lipoprotein
Antigen References
  1. Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press.
  2. Marzio R, et al. 1999. Immunopharmacol Immunotoxicol. 21:565-82.
Gene ID
969 View all products for this Gene ID
UniProt
View information about CD69 on UniProt.org
Go To Top Version: 1    Revision Date: 09.16.2024

For Research Use Only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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