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Cell-Vive™ GMP Ultra-LEAF™ Purified anti-human CD34 SF Antibody

Pricing & Availability
Clone
581 (See other available formats)
Other Names
Gp105-120, My10
Isotype
Mouse IgG1, κ
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Product Citations
publications
CV_GMP_ULEAF_CD34_SF_581_070723
Human myeloid cell line KG1a stained with Cell-Vive™ GMP Ultra-LEAF™ anti-human CD34 SF antibody (Clone 581, filled histogram) or isotype control (Clone MOPC-21, open histogram) followed by FITC anti-mouse IgG stain.
  • CV_GMP_ULEAF_CD34_SF_581_070723
    Human myeloid cell line KG1a stained with Cell-Vive™ GMP Ultra-LEAF™ anti-human CD34 SF antibody (Clone 581, filled histogram) or isotype control (Clone MOPC-21, open histogram) followed by FITC anti-mouse IgG stain.
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343548 1 mg 1980 CHF
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343547 100 µg 385 CHF
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Description

CD34, also known as gp105-120, is a type I monomeric sialomucin-like glycophosphoprotein with an approximate molecular weight of 105-120 kD. Selectively expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nervous tissue, CD34 is a commonly used marker to identify human hematopoietic stem/progenitor cells. According to the differential sensitivity to enzymatic cleavage, four groups of epitopes of CD34 have been described. CD34 mediates cell adhesion and lymphocytes homing through binding to L-selectin and E-selectin ligands.

Cell-Vive™ GMP Ultra-LEAF™ Purified anti-human CD34 SF Antibody was GMP manufactured under serum-free conditions including serum-free hybridoma cell culture, without additional animal or human-derived materials or preservatives. This antibody contains ultra-low levels of endotoxin (<0.01 EU/μg of protein), is filtered through a 0.1 μm membrane, and is tested negative for mycoplasma and microbial growths.

Cell-Vive™ GMP Ultra-LEAF™ Purified anti-human CD34 SF Antibody is for research and further ex vivo cell processing use only.

Product Details
Quality Statement

BioLegend Cell-Vive™ GMP Ultra-LEAF™ antibodies are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12 in a dedicated GMP facility compliant with ISO 13485:2016. Specifications and processes include:

  • Ultra-low endotoxin level (<0.01 EU/µg)
  • Manufactured under serum-free conditions
  • Bulks tested negative for mycoplasma and bacterial/fungal growth
  • Serum-free hybridomas are negative for the presence of murine virus
  • Batch-to-batch consistency
  • Vendor qualification
  • Raw material traceability and documentation
  • Documented procedures and employee training
  • Equipment maintenance and monitoring records
  • Lot-specific certificates of analysis
  • QA review of released products
  • Quality audits per ISO 13485:2016
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Reported Reactivity
Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
0.1 μm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
< 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test.
Preparation
Serum-Free hybridoma derived Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody purified by affinity chromatography.
Concentration
1.0 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
ICC, IHC-P, CyTOF® - Reported in the literature
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Cell-Vive™ GMP Ultra-LEAF™ Purified anti-human CD34 SF Antibody (Endotoxin < 0.01 EU/μg, Azide-Free, 0.1 μm filtered) is recommended for highly sensitive assays. The antibody was GMP manufactured under serum-free conditions including serum-free hybridoma cell culture, without additional animal or human-derived materials or preservatives. This antibody is intended for flow cytometry and for research or further ex vivo cell processing use only.

The 581 clone recognizes the class III group epitope which is resistant to sialidase/glycolyprotease and chymopapain treatment. Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections5 and immunofluorescence6.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman SF, et al. 1995. Leukocyte Typing V: White Cell Differentiation Antigen. New York: Oxford University Press.
  2. Felschow DM, et al. 2001. Blood. 97:3768.
  3. Rudin CE, et al. 1997. Br. J. Haematol. 97:488.
  4. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  5. Skowasch D, et al. 2003. Cardiovasc Res. 60:684. (IHC)
  6. Umland O, et al. 2003. J. Histochem. Cytochem. 51:977. (IF)
Disclaimer

BioLegend Cell-Vive™ GMP Ultra-LEAF™ antibodies are for research use only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

Antigen Details

Structure
105-120 kD single chain mucin-like glycoprotein
Distribution

Hematopoietic stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic fibroblasts

Function
Cell adhesion
Interaction
Endothelial cells, Fibroblasts, Hematopoietic stem, and progenitors
Ligand/Receptor
L-selectin, E-selectin
Antigen References
  1. Krause DS, et al. 1996. Blood. 87:1.
  2. Puri KD, et al. 1995. J. Cell Biol. 131:261.
  3. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules: The CD Markers. John Wiley & Sons Inc, Hoboken New Jersey.
Gene ID
947 View all products for this Gene ID
UniProt
View information about CD34 on UniProt.org
Go To Top Version: 3    Revision Date: 06.18.2024

For Research Use Only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at https://www.biolegend.com/terms (“Terms”) and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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