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Direct-Blot™ HRP anti-c-Myc Antibody

Pricing & Availability
Clone
9E10 (See other available formats)
Regulatory Status
RUO
Other Names
myc tag, c-Myc tag, cMyc tag, Myc epitope tag
Isotype
Mouse IgG1, κ
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Product Citations
publications
9E10_DirectBlotHRP_cmyc_Antibody_WB_021716
Recombinant Posi-Tag Epitope Tag Protein (1 µL, diluted 1:10) was resolved by electrophoresis, transferred to a nitrocellulose membrane, and probed with Direct-Blot™ HRP c-myc (clone 9E10). Proteins were visualized using chemiluminescence detection.
  • 9E10_DirectBlotHRP_cmyc_Antibody_WB_021716
    Recombinant Posi-Tag Epitope Tag Protein (1 µL, diluted 1:10) was resolved by electrophoresis, transferred to a nitrocellulose membrane, and probed with Direct-Blot™ HRP c-myc (clone 9E10). Proteins were visualized using chemiluminescence detection.
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626804 25 µL 101 CHF
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626803 100 µL 288 CHF
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Description

The 9E10 monoclonal antibody recognizes the amino acid sequence EQKLISEEDL, which is a specific portion of the human c-myc gene product. This antibody is highly specific and is unlikely to alter the activity of the cloned sequence.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Epitope tag
Reported Reactivity
Species independent
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Amino acids 408-439, C-terminal region of human c-myc
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:2000. The optimal dilution should be determined by titration for each individual assay of interest.

25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.

Application Notes

*Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.

Application References

(PubMed link indicates BioLegend citation)
  1. Rao H, et al. 2001. Nature. 410:955.
  2. Peters CS, et al. 2001. J Biol Chem. 276:13718.
  3. Munro S, Pelham HR. 1987. Cell. 48:899.
  4. Evan GI, et al. 1985. Mol Cell Biol. 5:3610.
Product Citations
  1. Calvo IA, et al. 2021. iScience. 24:103338. PubMed
RRID
AB_2572009 (BioLegend Cat. No. 626804)
AB_2572009 (BioLegend Cat. No. 626803)

Antigen Details

Biology Area
Cell Biology, Protein Purification, Protein Synthesis
Antigen References
  1. Adams JM, et al. 1983. Proc. Natl. Acad. Sci. USA 80:1982.
  2. Atchley WR, et al. 1995. Proc. Natl. Acad. Sci. USA 92:10217.
  3. Battey J, et al. 1983. Cell 34:779.
  4. Beimling P, et al. 1985. Biochemistry 24:6349.
Gene ID
4609 View all products for this Gene ID
UniProt
View information about c-Myc on UniProt.org
Go To Top Version: 5    Revision Date: 10.03.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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