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Direct-Blot™ HRP anti-STAT5 Antibody

Pricing & Availability
Clone
9C8B50 (See other available formats)
Regulatory Status
RUO
Other Names
Signal transducer and activator of transcription 5A
Isotype
Mouse IgG2a, κ
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Product Citations
publications
9C8B50_DBHRP_STAT5_Antibody_WB_062116
10 µg of total protein extract from Jurkat cells was resolved by electrophoresis, transferred to a nitrocellulose membrane, and probed with Direct-Blot™ HRP anti-STAT5 (clone 9C8B50) antibody. Proteins were visualized using chemiluminescence detection.
  • 9C8B50_DBHRP_STAT5_Antibody_WB_062116
    10 µg of total protein extract from Jurkat cells was resolved by electrophoresis, transferred to a nitrocellulose membrane, and probed with Direct-Blot™ HRP anti-STAT5 (clone 9C8B50) antibody. Proteins were visualized using chemiluminescence detection.
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660203 100 µL 340 CHF
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Description

STAT5 is a member of the signal transducer and activator of transcription factors (STAT) family, mediating growth and cytokine signaling. STAT5 consists of two closely related family members, STAT5A and STAT5B, which exhibit 96% sequence homology and are functionally redundant.  Upon activation, STAT5 is phosphorylated by receptor tyrosine kinases and, in turn, forms homodimers or hererodimers with other family members through its SH2 domains. The dimerized STAT5 translocates to the nucleus and binds to the STAT5 response element (TTCXXXGAA). STAT5 plays crucial regulatory roles in cell proliferation, anti-apoptosis, early B cell differentiation, and T cell development.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human STAT5A recombinant protein (627-794 a.a.) expressed in E. coli.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:4000. The optimal dilution should be determined by titration for each individual assay of interest.

25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.

Application Notes

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

RRID
AB_2616893 (BioLegend Cat. No. 660203)

Antigen Details

Structure
794 amino acids, predicted molecular weight of 91 kD; contains a SH2 domains responsible for homodimerization or heterodimerization.
Distribution

Cytoplasm; phosphorylated form translocates to the nucleus.

Function
STAT5 is a transcription regulator and is involved in cell differentiation, proliferation, and survival, in response to several cytokines, such as interleukins, erythropoietin, growth hormone, thrombopoietin, and prolactin.
Interaction
Forms a homodimer or a heterodimer with other family members. Interacts with NCOA1, SOCS7, CBP, p300, SMRT and ERBB4.
Cell Type
B cells, Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Signal Transduction, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Bell BD, et al. 2013. Nat. Immunol. 14:364.
2. Cholez E, et al. 2012. Leukemia. 26:2390.
3. Heltemes-Harris LM, et al. 2012. Curr. Opin. Immunol. 24:146.
4. Heltemes-Harris LM, et al. 2011. Ann. N Y Acad. Sci. 1217:18.
5. Malin S, et al. 2010. Curr. Opin. Immunol. 22:168.
6. Xu J, et al. 2009. J. Neurosci. 29:2022.

Gene ID
6776 View all products for this Gene ID
UniProt
View information about STAT5 on UniProt.org
Go To Top Version: 2    Revision Date: 06.21.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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