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ELISA MAX™ Deluxe Set Human Phospho-EGFR (Tyr845)

Pricing & Availability
Regulatory Status
RUO
Other Names
Human Phospho EGFR (Y 845), Human EGFR (pTyr845), Human EGFR (pY845)
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Product Citations
publications
ELISA-MAX_Deluxe_Human_Phospho-EGFR_Tyr845_071323
ELISA MAX™ Deluxe Set Human Phospho-EGFR (Tyr845) Standard Curve
  • ELISA-MAX_Deluxe_Human_Phospho-EGFR_Tyr845_071323
    ELISA MAX™ Deluxe Set Human Phospho-EGFR (Tyr845) Standard Curve
  • ELISA-MAX_Deluxe_Human_Phospho-EGFR_Tyr845_2_071323.
    The quantification of Human Phospho-EGFR (Tyr845) is demonstrated by treating A431 cells with or without the EGFR kinase inhibitor PD153035. A431 cells were incubated with or without recombinant human EGF (Cat. No. 585506) at 100 ng/mL after being pre-treated with or without the EGFR-kinase inhibitor PD153035 at either 10 nM, 20 nM, 50 nM, or 100 nM. Cells were collected and then lysed with ice-cold lysis buffer. BCA assay was performed to measure total protein concentration. All lysate samples were diluted down to 50 µg/mL total protein in 1X ADB before being analyzed with the ELISA MAX™ Deluxe Set Human Phospho-EGFR (Tyr845) assay. Then, the measured phospho-EGFR (Tyr845) was normalized to the total protein concentration.
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449804 5 plates 645 CHF
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Description

EGFR (Epidermal Growth Factor Receptor) is a member of the ErbB family of cell surface receptors. It is an oncoprotein which has been found to have increased expression and activity in multiple cancer types. However, low activity of EGFR can result in degenerative diseases such as Alzheimer’s disease. EGFR signaling pathway starts when it binds to the ligand EGF, resulting in receptor dimerization, transphosphorylation of the C-terminal tails, and propagation of the signal downstream. Each phosphorylation site can serve as a docking site for different proteins of different signaling pathways. Phosphorylation of tyrosine 845 is associated with regulation of receptor function and tumor progression via c-Src in a Zn2+-dependent manner.

BioLegend’s ELISA MAX™ Deluxe Set contains the components necessary for the accurate quantification of natural and recombinant human Phospho-EGFR (Tyr845). The set is designed for cost-effective and accurate quantification of human Phospho-EGFR (Tyr845) in cell and tissue lysates. BioLegend’s ELISA MAX™ Deluxe Sets are sensitive, accurate, and robust.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Human Phospho-EGFR (Tyr845) ELISA MAX™ Capture Antibody (200X)
  • Human Phospho-EGFR (Tyr845) ELISA MAX™ Detection Antibody (200X)
  • Human Phospho-EGFR (Tyr845) Lyophilized Standard
  • Avidin-HRP (1000X)
  • Substrate Solution F
  • Coating Buffer A (5X)
  • Assay Diluent B (5X)

Product Details

Verified Reactivity
Human
Application

ELISA
 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Sensitivity
0.064 ± 0.036 ng/mL
Standard Range
0.16 - 10 ng/mL
Materials Not Included
  • Microwell plates: BioLegend Cat. No. 423501 is recommended
  • Wash Buffer: BioLegend Cat. No. 421601 is recommended, or PBS + 0.05% Tween-20
  • Stop Solution: BioLegend Cat. No. 423001 is recommended, or acid solution, e.g. 2N H2SO4
  • Plate Sealers: BioLegend Cat. No. 423601 is recommended
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 μm filtered
  • Deionized (DI) water
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 μL to 1 mL
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer

Antigen Details

Cell Type
Epithelial cells
Biology Area
Cancer Biomarkers, Cell Biology, Immuno-Oncology, Immunology, Signal Transduction
Molecular Family
Phospho-Proteins, Protein Kinases/Phosphatase
Gene ID
NA
UniProt
View information about EGFR Tyr845 on UniProt.org

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 1    Revision Date: 07.13.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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