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ELISA MAX™ Deluxe Set Human PLAUR (uPAR)

Pricing & Availability
Regulatory Status
RUO
Other Names
CD87, MO3, Plasminogen activator urokinase receptor, uPAR, URKR
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ELISA-MAX_Deluxe_Human_PLAUR-uPAR_1_080422
  • ELISA-MAX_Deluxe_Human_PLAUR-uPAR_1_080422
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448404 5 plates 373 CHF
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Description

PLAUR (uPAR), also known as CD87, is a urokinase-type plasminogen activator system consisting of proteinase, receptor, and inhibitor. PLAUR is involved
in proteolysis, cell migration and tissue remodeling by proteolysis, signal transduction and chemokine-like activities. It predominately plays roles in cell activation, adhesion, migration, and extravasation in blood cells.

High serum levels of soluble PLAUR (uPAR) are associated with lower survival rates in cancer and HIV-1 patients. Research on COVID-19 suggests that levels of PLAUR (uPAR) are increased in COVID-19 patients that are hospitalized and are in critical condition.

The BioLegend ELISA MAX™ Deluxe Set is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA). The mouse monoclonal capture antibody is first coated on a 96-well plate. Standards and samples are added to the wells and Human PLAUR (uPAR) protein binds to the immobilized capture antibody. Next, biotinylated anti-human PLAUR (uPAR) detection antibody is added, producing an antibody-antigen-antibody “sandwich”. Avidin horseradish peroxidase is subsequently added, followed by TMB Substrate Solution, producing a blue color in proportion to the concentration of Human PLAUR (uPAR) present in the sample. Finally, the Stop Solution changes the reaction color from blue to yellow, and the microwell absorbance is read at 450 nm with a microplate reader.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Human PLAUR (uPAR) ELISA MAX™ Capture Antibody (200X)
  • Human PLAUR (uPAR) ELISA MAX™ Detection Antibody (200X)
  • Human PLAUR (uPAR) Standard
  • Avidin-HRP (1000X)
  • Substrate Solution D
  • Coating Buffer B (5X)
  • Assay Diluent B (5X)

Product Details

Verified Reactivity
Human
Application

ELISA
 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Sensitivity
0.57 ± 0.43 pg/mL
Standard Range
3.9 – 250 pg/mL
Materials Not Included
  • Microwell plates: BioLegend Cat. No. 423501 is recommended
  • Wash Buffer: BioLegend Cat. No. 421601 is recommended, or PBS + 0.05% Tween-20
  • Stop Solution: BioLegend Cat. No. 423001 is recommended, or acid solution, e.g. 2N H2SO4
  • Plate Sealers: BioLegend Cat. No. 423601 is recommended
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 μm filtered
  • Deionized (DI) water
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 μL to 1 mL
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer

Antigen Details

Molecular Family
Soluble Receptors
Gene ID
NA
UniProt
View information about PLAUR on UniProt.org

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 1    Revision Date: 08.04.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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