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FITC anti-human CD100 Antibody

Pricing & Availability
Clone
A8 (See other available formats)
Regulatory Status
RUO
Workshop
V BP383, S233
Other Names
Sema4D
Isotype
Mouse IgG1, κ
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Product Citations
publications
A8_FITC_090808.jpg
Human peripheral blood lymphocytes stained with A8 FITC
  • A8_FITC_090808.jpg
    Human peripheral blood lymphocytes stained with A8 FITC
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328406 100 tests 229 CHF
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Description

CD100, a 150 kD homodimer, is a novel semaphorin that induces B cells to aggregate and improves their viability in vitro. Semaphorins are neuronal chemorepellants that direct pioneering neurons during nervous system development. CD100 modifies CD40-CD40L B cell signaling by augmenting B cell aggregation and survival and down regulating CD23 expression. CD72 is a lymphocyte receptor for the class IV semaphorin CD100, which is involved in regulating B cell signaling. CD100 is expressed on resting and PHA stimulated T cells. The protein is weakly expressed on NK cells, EBV transformed B cells, monocytes and tumor/peripheral blood T cell lines, and at higher density on activated T cells and B cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human PHA activated lymphocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Hall K, et al. 1996. P. Natl. Acad. Sci. USA 93:11780.
  2. Mizrahi S, et al. 2007. PLoS One. 2(9):e818.
  3. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Product Citations
  1. Grandclaudon M, et al. 2019. Cell. 179:432. PubMed
RRID
AB_2254362 (BioLegend Cat. No. 328406)

Antigen Details

Structure
Class IV semaphorin family member, 150 kD homodimer
Distribution

T cells, B cells, weak on NK cells

Function
Costimulatory molecule in adaptive immune responses that switches off negative signaling from CD72
Cell Type
B cells, NK cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Schlossman SL Bloumsell W Gilks et al. eds. 1995. Leucocyte Typing V:White Cell Differentiation Antigens. Oxford University Press New York.
2. Bougeret CIG Mansur H Dastot et al. 1992. Increased surface expression of a newly identified 150 kDa dimer early after

Gene ID
10507 View all products for this Gene ID
UniProt
View information about CD100 on UniProt.org
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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