FITC anti-human CD40 Recombinant Antibody, CD40, QA18A47

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Clone: QA18A47 (See other available formats)
Regulatory Status: RUO
Other Names: BP50, TNFRSF5
Isotype: Mouse IgG1, κ
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Product Citations: publications

QA18A47_FITC_CD40_Antibody_061623 — Human peripheral blood lymphocytes were stained with anti-human CD19 APC and anti-human CD40 recombinant (clone QA18A47) FITC (left) or mouse IgG1, κ FITC isotype control (right).

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325809

25 tests

90 CHF

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325810

100 tests

195 CHF

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Description

CD40 is a 48 kD type I glycoprotein also known as BP50. It is a member of the TNFR superfamily primarily expressed on B cells, macrophages, follicular dendritic cells, endothelial cells, fibroblasts, and at low levels on plasma cells. CD40 has been reported to be involved in B cell differentiation, costimulation, isotype class-switching, and protection of B cells from apoptosis. Additionally, CD40 is important for T cell-B cell interactions. The ligand of CD40 is CD154 (CD40 ligand). The QA18A46 antibody has been reported to promote B cell proliferation in the presence of anti-IgM, IL-4 or PMA, partially blocking CD40 binding to CD40L, and B cells rescue from apoptosis.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Recombinant
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Clone QA18A47 can completely block the binding of clone 5C3 on target cells.
RRID: AB_3068118 (BioLegend Cat. No. 325809)
AB_3068118 (BioLegend Cat. No. 325810)

Antigen Details

Structure: TNFR superfamily, type I glycoprotein, 48 kD
Distribution: B cells, macrophages, follicular dendritic cells, endothelial cells, fibroblasts
Function: B cell differentiation, costimulation, isotype class-switching, rescues B cells from apoptosis
Interaction: TRAF signaling molecules
Ligand/Receptor: CD154 (CD40 ligand)
Cell Type: Antigen-presenting cells, B cells, Dendritic cells, Macrophages
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Banchereau J, et al. 1994. Annu. Rev. Immunol. 12:881.
2. Foy T, et al. 1996. Annu. Rev. Immunol. 14:591.
Gene ID: 958 View all products for this Gene ID
UniProt: View information about CD40 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All CD40 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD40 Recombinant Antibody	QA18A47	FC
APC anti-human CD40 Recombinant Antibody	QA18A47	FC
PE anti-human CD40 Recombinant Antibody	QA18A47	FC
PE/Cyanine7 anti-human CD40 Recombinant Antibody	QA18A47	FC
FITC anti-human CD40 Recombinant Antibody	QA18A47	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 06.16.2023

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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