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FITC anti-human CD46 Antibody

Pricing & Availability
Clone
MEM-258 (See other available formats)
Regulatory Status
RUO
Other Names
Complement membrane cofactor protein, measles virus receptor, MCP
Isotype
Mouse IgG1, κ
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Product Citations
publications
MEM-258_FITC_062707
Human peripheral blood lymphocytes stained with MEM-258 FITC
  • MEM-258_FITC_062707
    Human peripheral blood lymphocytes stained with MEM-258 FITC
See FITC spectral data
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315304 100 tests 229 CHF
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Description

CD46 is a transmembrane protein also known as complement membrane cofactor protein, MCP, and measles virus receptor. It is widely expressed on leukocytes, platelets, epithelial cells and fibroblasts. Multiple isoforms of CD46 have been reported with molecular weights ranging from 45-75 kD; CD46 binds complement components C3b and C4b and has also been reported to play a role in T cell regulation. In addition to the complement components, CD46 has been shown to interact with moesin, c-Src, tetraspanin 4, c-Yes, and integrin β1. CD46 is also known to be a cellular receptor for human measles virus and human herpes virus-6. Defects in CD46 have been associated with hemolytic-uremic syndrome. The MEM-258 antibody has been shown to be useful for flow cytometry, Western blotting, and immunoprecipitation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
HPB-ALL T cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Application Notes

Clone MEM-258 recognizes an epitope on the SCR4 (membrane-proximal SCR) domain of CD46. Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting (non-reducing).

Application References

(PubMed link indicates BioLegend citation)
  1. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
  2. Kickler K, et al. 2012. J immunol. 188:5303. PubMed
  3. Bach P, et al. 2013. Cancer Res. 73:865. PubMed
  4. Wu Y, et al. 2014. Am J Physiol Gastrointest Liver Physiol. 306:1056. PubMed
Product Citations
  1. Wohlgemuth L, et al. 2022. Cells. 11: . PubMed
  2. Bach P, et al. 2013. Cancer Res. 73:865. PubMed
  3. Davies L, et al. 2016. Stem Cells Trans Med. 5: 1485 - 1495. PubMed
  4. Kickler K, et al. 2012. J Immunol. 188:5303. PubMed
  5. Tritschler H, et al. 2021. Cells. 10:. PubMed
  6. Ammour Y, et al. 2020. Viruses. 12:. PubMed
  7. Wu Y, et al. 2014. Am J Physiol Gastrointest Liver Physiol. 306:1056. PubMed
RRID
AB_2291290 (BioLegend Cat. No. 315304)

Antigen Details

Structure
Transmembrane receptor, contains 4 SUSHI domains, multiple isoforms exist ranging in molecular weight from 45-75 kD. The larger isoform 65-75 kD is not expressed on peripheral blood cells.
Distribution
Widely expressed in leukocytes, platelets, epithelial cells and fibroblasts
Function
Binds complement components C3B and C4B with cofactor activity for the I-dependent cleavage of these proteins. May also play a role in T cell regulation
Interaction
Moesin, c-Src, complement component 3, complement component 4B, tetraspanin 4, c-Yes, integrin β1
Ligand/Receptor
C3B, C4B, Measles virus, human herpesvirus-6
Modification
Phosphorylation (Y368)
Cell Type
Leukocytes, Platelets, Epithelial cells, Fibroblasts
Biology Area
Cell Biology, Complement, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Dorig RE, et al. 1993. Cell 75:295.
2. Cui W, et al. 1993. J. Immunol. 151:4137.
3. Kemper C, et al. 2003. Nature 421:388.
4. Marie JC, et al. 2002. Nat. Immunol. 3:659.

Gene ID
4179 View all products for this Gene ID
UniProt
View information about CD46 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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