FITC anti-human CD64 Antibody, CD64, S18012E

Pricing & Availability

Clone: S18012E (See other available formats)
Regulatory Status: RUO
Other Names: FcγRI, FcR I
Isotype: Mouse IgG1, κ
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Product Citations: publications

S18012E_FITC_CD64_Antibody_033120 — Human peripheral blood monocytes were stained with CD64 (clone S18012E) FITC (filled histogram) or mouse IgG1, κ (clone MOPC-21) FITC isotype control (open histogram).

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399605

25 tests

83 CHF

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399606

100 tests

188 CHF

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Description

CD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcR I. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and activated granulocytes. The expression can be upregulated by IFN-γ stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human CD64-Fc (1257-FC-050)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Application Notes: In-house testing suggests that S18012E partially blocks the binding of clone 10.1 but not clone S18012C. Clone 10.1 recognizes the EC3 epitope of CD64. While both CD64 subunits contain EC3 domains, clone 10.1 preferentially binds to CD64A (FcγRIA) but not CD64B (FcγRIB).
RRID: AB_2861011 (BioLegend Cat. No. 399605)
AB_2861011 (BioLegend Cat. No. 399606)

Antigen Details

Structure: Ig superfamily, type I glycoprotein, 72 kD
Distribution: Monocytes, macrophages, dendritic cells, activated granulocytes
Function: Phagocytosis, ADCC
Ligand/Receptor: IgG receptor
Cell Type: Dendritic cells, Granulocytes, Macrophages, Monocytes
Biology Area: Immunology, Inhibitory Molecules
Molecular Family: CD Molecules, Fc Receptors
Antigen References: Hulett MD & Hogarth PM. 1994. Adv Immunol. 57:1-127.

van de Winkel JG & Capel PJ. 1993. Immunol Today. 14:215-21.
Gene ID: 2209 View all products for this Gene ID
UniProt: View information about CD64 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All CD64 Reagents Request Custom Conjugation

Description	Clone	Applications
FITC anti-human CD64	S18012E	FC
PE anti-human CD64	S18012E	FC
Purified anti-human CD64	S18012E	FC
PE/Dazzle™ 594 anti-human CD64	S18012E	FC
PerCP/Cyanine5.5 anti-human CD64	S18012E	FC
APC/Fire™ 810 anti-human CD64 Antibody	S18012E	FC
PE/Cyanine7 anti-human CD64	S18012E	FC
Alexa Fluor® 700 anti-human CD64	S18012E	FC
APC anti-human CD64	S18012E	FC
APC/Fire™ 750 anti-human CD64	S18012E	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 03.31.2020

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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