FITC anti-mouse CD45 antibody, CD45, S18009F

Pricing & Availability

Clone: S18009F (See other available formats)
Regulatory Status: RUO
Other Names: T200, Ly-5, LCA
Isotype: Rat IgG2b, κ
Ave. Rating: Submit a Review
Product Citations: publications

C57BL/6 mouse splenocytes were stained with anti-mouse CD45 (clone S18009F) FITC (filled histogram) or FITC rat IgG2b, κ isotype control (open histogram).

Compare all formats See FITC spectral data

Cat #

Size

Price

Quantity Check Availability

Save

157213

25 µg

30 CHF

Check Availability

Need larger quantities of this item?
Request Bulk Quote

157214

100 µg

77 CHF

Check Availability

Need larger quantities of this item?
Request Bulk Quote

Description

CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from variable splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant mouse CD45
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
RRID: AB_2894427 (BioLegend Cat. No. 157213)
AB_2894427 (BioLegend Cat. No. 157214)

Antigen Details

Structure: Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution: All hematopoietic cells except mature erythrocytes and platelets
Function: Phosphatase, T and B cell activation
Interaction: Mesenchymal Stem Cells, B cells, Dendritic cells, Tregs
Ligand/Receptor: Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Biology Area: Cell Biology, Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.

Trowbridge IS, et al. 1994. Annu. Rev. Immunol. 12:85.

Kishihara K, et al. 1993. Cell. 74:143.

Pulido R, et al. 1988. J. Immunol. 140:3851.
Gene ID: 19264 View all products for this Gene ID
UniProt: View information about CD45 on UniProt.org

Documentation

Reviews

Review This Product

Related Protocols

Cell Surface Flow Cytometry Staining Protocol

Related Products

Description	Clone	Applications

Related Pages & Pathways

Pathways

B-Cell Receptor Pathway

Related FAQs

Other Formats

View All CD45 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-mouse CD45	S18009F	FC
APC/Cyanine7 anti-mouse CD45	S18009F	FC
PE/Cyanine7 anti-mouse CD45	S18009F	FC
PerCP/Cyanine5.5 anti-mouse CD45	S18009F	FC
Alexa Fluor® 700 anti-mouse CD45	S18009F	FC
Pacific Blue™ anti-mouse CD45	S18009F	FC
PE/Fire™ 810 anti-mouse CD45	S18009F	FC
FITC anti-mouse CD45	S18009F	FC
Brilliant Violet 605™ anti-mouse CD45	S18009F	FC
Brilliant Violet 510™ anti-mouse CD45	S18009F	FC

Certificate of Analysis

Customers Also Purchased

APC anti-mouse CD45

Clone: I3/2.3

PE anti-mouse CD4

Clone: GK1.5

Brilliant Violet 605™ anti-mouse CD45

Clone: 30-F11

FITC anti-human CD45

Clone: HI30

Go To Top Version: 1 Revision Date: 09.15.2021

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.