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FITC anti-p53 Antibody

Pricing & Availability
Clone
DO-7 (See other available formats)
Regulatory Status
RUO
Other Names
Tumor protein p53 (TP53)
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
DO-7_FITC_p53_Antibody_020813
A431 cells, which are a human epidermoid carcinoma cell line, was fixed and permeabilized with FOXP3 Fix/Perm Buffer Set, and then stained with p53 (clone DO-7) FITC (filled histogram) or mouse IgG2b, κ FITC isotype control (open histogram).
  • DO-7_FITC_p53_Antibody_020813
    A431 cells, which are a human epidermoid carcinoma cell line, was fixed and permeabilized with FOXP3 Fix/Perm Buffer Set, and then stained with p53 (clone DO-7) FITC (filled histogram) or mouse IgG2b, κ FITC isotype control (open histogram).
Compare all formats See FITC spectral data
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645803 25 tests 129 CHF
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645804 100 tests 288 CHF
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Description

p53 is a 53 kD protein that forms tetramers and functions as a tumor suppressor and transcriptional activator of genes that inhibit growth and/or invasion, cell cycle checkpoint after irradiation, DNA repair, apoptotic induction, signal transduction, and cell adhesion. This protein is localized to the nucleus when activated and can be upregulated by genotoxic or other cellular stresses. p53 is modified by phosphorylation, acetylation, ribosylation, ubiquitination, and sumoylation; ubiquination targets p53 for degradation via mdm2. This protein interacts with a variety of proteins including mdm2, mdmx, topoisomerase I, PML3, Bcl-XL, Bcl-2, Chk1, JNK, p38, HIPK2, CK2, DNA-PK, p300/CBP, PCAF, PARP1, and HDAC1-3. Mutant p53 associates with p63 and p73.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
p53 N-terminal linear epitope aa 20-25
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Vojtesek B, et al. 1992. J. Immunol. Methods 151:237.
  2. Stephen CW, et al. 1995. J. Mol. Biol. 248:58.
Product Citations
  1. Dong Y, et al. 2020. J Leukoc Biol. 108:1711. PubMed
  2. Barili V, et al. 2020. Nat Commun. 0.877777778. PubMed
  3. Ladds MJGW, et al. 2018. Nat Commun. 9:1107. PubMed
  4. Kannan S, et al. 2019. Mol Cancer Ther. 1.871527778. PubMed
RRID
AB_2561798 (BioLegend Cat. No. 645803)
AB_2561798 (BioLegend Cat. No. 645804)

Antigen Details

Structure
Tetramer; 53 kD
Distribution

Nuclear when activated

Function
Tumor suppressor, transcription factor regulates cell cycle checkpoint, apoptosis, DNA integrity
Interaction
mdm2, mdmx, TopoI, PML3, Bcl-XL, Bcl-2, Chk1, JNK, p38, HIPK2, CK2, DNA-PK, p300/CBP, PCAF, PARP1, HDAC1-3; mutant p53 associates with p63, p73
Cell Type
Mesenchymal Stem Cells
Biology Area
Cell Biology, DNA Repair/Replication, Stem Cells
Gene ID
7157 View all products for this Gene ID
UniProt
View information about p53 on UniProt.org

Other Formats

View All p53 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-p53 DO-7 WB,ICC,ChIP,ICFC,ELISA
FITC anti-p53 DO-7 ICFC
PE anti-p53 DO-7 ICFC
Go To Top Version: 2    Revision Date: 06.14.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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