FITC anti-rat CD45RA Antibody anti-CD45RA

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Clone: OX-33 (See other available formats)
Regulatory Status: RUO
Other Names: Leukocyte common antigen, high molecular weight form
Isotype: Mouse IgG1, κ
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Product Citations: publications

OX-33_FITC_042408 — LOU rat splenocytes stained with OX-33 FITC

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202305

100 µg

153 CHF

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Description

CD45 is a protein tyrosine phosphatase with multiple isoforms differing as a result of alternative splicing of the extracellular domain and glycosylation. CD45 is expressed on all hematopoietic cells except erythrocytes and platelets. CD45RA is one of the CD45 isoforms with a molecular weight of 200-220 kD. It is expressed almost exclusively on B cells. CD45 functions in signal transduction through T and B cell antigen receptors. CD45 has been shown to interact with various proteins, including galectin-1, CD2, CD3, and CD4.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Leukocyte common antigen purified from rat splenocytes.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections¹.
RRID: AB_314009 (BioLegend Cat. No. 202305)

Antigen Details

Structure: Protein tyrosine phosphatase, type I transmembrane protein, hign molecular weight isoform approximately 200-220 kD
Distribution: Primarily on B lymphocytes
Function: B cell receptor signal transduction
Ligand/Receptor: Galectin-1, CD2, CD3, CD4
Cell Type: B cells
Biology Area: Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules
Antigen References: 1. Sunderland CA, et al. 1979. Eur. J. Immunol. 9:155.
2. Woolett GR, et al. 1985. Eur. J. Immunol. 15:168.
Gene ID: 24699 View all products for this Gene ID
UniProt: View information about CD45RA on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Pathways

Stem Cells and Development

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Other Formats

View All CD45RA Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-rat CD45RA	OX-33	FC,IHC-F
Biotin anti-rat CD45RA	OX-33	FC
FITC anti-rat CD45RA	OX-33	FC
PE anti-rat CD45RA	OX-33	FC
Alexa Fluor® 488 anti-rat CD45RA	OX-33	FC
APC anti-rat CD45RA	OX-33	FC
PerCP/Cyanine5.5 anti-rat CD45RA	OX-33	FC
PE/Cyanine7 anti-rat CD45RA	OX-33	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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