FITC anti-rat CD62L Antibody anti-CD62L

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Clone: OX-85 (See other available formats)
Regulatory Status: RUO
Other Names: LECAM-1, Ly-22, LAM-1
Isotype: Mouse IgG1, κ
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Product Citations: publications

LOU rat splenocytes stained with OX-85 FITC

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202905

100 µg

153 CHF

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Description

CD62L is a 74-95 kD glycoprotein,also known as L-selectin, LECAM-1, Ly-22 and MEL-14.It is a member of the selectin family and is expressed on the majority of B and naïve T cells,a subset of memory T cells,granulocytes ,subsets of thymocytes and NK cells.CD62L is important in lymphocyte homing to high endothelial venules in peripheral lymph nodes and leukocyte “rolling” on activated endothelium. CD62L also contributes to neutrophil emigration at inflammatory sites.CD62L is rapidly shed from lymphocytes and neutrophils upon activation and the expression levels of CD62L (in conjunction with other markers) hae been used to distinguish naïve, effector and memory T cells.CD62L has been reported to interact with )-glycans presented on CD34, GlyCAM-1, and MadCAM-1.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Rat purified CD62L-CD4
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
RRID: AB_1089048 (BioLegend Cat. No. 202905)

Antigen Details

Structure: 74-95 Kd glycoprotein of selectin family.
Distribution: Expressed on majority of B cell and naïve T cell and other leukocytes
Function: Homing and emigration of leukocytes
Cell Type: B cells, T cells, Leukocytes
Biology Area: Immunology, Costimulatory Molecules
Molecular Family: Adhesion Molecules, CD Molecules
Regulation: Decreased expression upon cell activation
Gene ID: 20343 View all products for this Gene ID
UniProt: View information about CD62L on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

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Other Formats

View All CD62L Reagents Request Custom Conjugation

Description	Clone	Applications
FITC anti-rat CD62L	OX-85	FC
PE anti-rat CD62L	OX-85	FC
PerCP anti-rat CD62L	OX-85	FC
APC anti-rat CD62L	OX-85	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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