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FITC anti-rat CD90 Antibody

Pricing & Availability
Clone
2E11 (See other available formats)
Regulatory Status
RUO
Other Names
Thy-1, Thy1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
2E11_FITC_CD90_Antibody_FC_092215
LOU rat thymocytes were stained with rat CD90 (clone 2E11) FITC (filled histogram) or mouse IgG1, κ FITC isotype control (open histogram).
  • 2E11_FITC_CD90_Antibody_FC_092215
    LOU rat thymocytes were stained with rat CD90 (clone 2E11) FITC (filled histogram) or mouse IgG1, κ FITC isotype control (open histogram).
Compare all formats See FITC spectral data
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206105 50 µg 65 CHF
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Description

CD90, also known as Thy-1, is a 28-30 kD GPI-linked membrane glycoprotein. It is a member of the immunoglobulin superfamily and has been shown to interact with CD45 in signal transduction during lymphocyte proliferation and differentiation. CD90 is expressed on hematopoietic stem cells, neurons, thymocytes, peripheral T cells, fibroblasts, and stromal cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
PC12 cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for relevant formats of this clone) include: immunoprecipitation and Western blotting.

For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 206104) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg)

Application References

(PubMed link indicates BioLegend citation)
  1. Kuroiwa K, et al. 2012. Hybridoma 31:225.
Product Citations
  1. Volkova MV, et al. 2023. Int J Mol Sci. 24:. PubMed
  2. Wang L, et al. 2023. Pharm Biol. 61:568. PubMed
  3. El-Husseiny HM, et al. 2023. Int J Mol Sci. 24:. PubMed
  4. Yang F, et al. 2023. Mol Med Rep. 28:. PubMed
  5. Han X, et al. 2023. Stem Cells Int. 2023:8992284. PubMed
  6. Lan WR, et al. 2019. Stem Cells Int. 2019:8404168. PubMed
  7. Chou PR, et al. 2020. Int J Med Sci. 17:354. PubMed
  8. Qing P, et al. 2020. Exp Physiol. 1708:105. PubMed
  9. Shi Z, et al. 2020. Stem Cell Res Ther. 0.634027778. PubMed
  10. Sun J, et al. 2018. Exp Ther Med. 15:1424. PubMed
  11. Wei ZJ, et al. 2019. Neural Regen Res. 14:1462. PubMed
RRID
AB_2566265 (BioLegend Cat. No. 206105)

Antigen Details

Structure
Ig superfamily, GPI-anchored membrane glycoprotein.
Distribution

Hematopoietic stem cells, thymocytes, a subset of peripheral blood T cells, early myeloid and erythroid progenitors, immature B cells, neurons, activated endothelium, mast cells, dendritic cells, and glomeruli.

Function
Adhesion, signal transduction, lymphocyte co-stimulation, and proliferation and differentiation of hematopoietic stem cells.
Ligand/Receptor
Interacts with CD45.
Cell Type
B cells, Dendritic cells, Hematopoietic stem and progenitors, Mast cells, Neurons, T cells, Thymocytes
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Campbell DG, et al. 1981. Biochem. J. 195:15.
2. Hosseinzadeh H, et al. 1993. J. Immunol. 150:1670.

Gene ID
21838 View all products for this Gene ID
UniProt
View information about CD90 on UniProt.org
Go To Top Version: 1    Revision Date: 09.22.2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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