GMP APC anti-human CD94 Antibody

Pricing & Availability
Clone
DX22 (See other available formats)
Other Names
KP43
Isotype
Mouse IgG1, κ
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Product Citations
publications
DX22_GMP_APC_CD94_Antibody_082024
Typical results from human peripheral blood lymphocytes stained either with DX22 APC used at 5 µL/test (filled histogram) or with an isotype control (open histogram).
  • DX22_GMP_APC_CD94_Antibody_082024
    Typical results from human peripheral blood lymphocytes stained either with DX22 APC used at 5 µL/test (filled histogram) or with an isotype control (open histogram).
Compare all formats See GMP APC spectral data
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260386 100 tests 347 CHF
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Description

CD94 is a 43 kD type II transmembrane glycoprotein also known as KP43. CD94 belongs to the C-type lectin superfamily and is present as a covalently linked heterodimer with NKG2 on the cell surface. CD94 is expressed by NK cells, a subset of γδ T cells, and NKT cells. The CD94/NKG2A complex serves as an inhibitory receptor specific for HLA-class I molecules.

Product Details
Quality Statement This BioLegend’s GMP-RUO reagent is a fluorochrome-labeled monoclonal antibody single reagent for Research Use Only. Suitable for use in laboratory-developed multicolor flow cytometry tests. Single GMP-RUO products can be used in various multicolor flow cytometry combinations. Laboratory need to establish the GMP-RUO performance characteristics in combination with other reagents in normal and pathological samples.
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
NK cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA) and a stabilizer.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
25 µg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation4, inhibition of NK cell-mediated lysis5, immunohistochemical staining of acetone-fixed frozen tissue sections, and spatial biology (IBEX)6,7.

Application References

(PubMed link indicates BioLegend citation)
  1. Mizuki M, et al. 2000. Sarcoidosis Vasc. Diffuse Lung Dis. 17:54.
  2. Phillip J, et al. 1996. Immunity 5:163.
  3. Lazetic S, et al. 1996. J. Immunol. 157:4741.
  4. Lanier LL, et al. 1998. Immunity 8:693.
  5. Wooden SL, et al. 2005. J. Immunol. 175:1383.
  6. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  7. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Disclaimer

GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include:

  • Batch-to-batch consistency
  • Material traceability
  • Documented procedures
  • Documented employee training
  • Equipment maintenance and monitoring records
  • Lot-specific certificates of analysis
  • Quality audits per ISO 13485:2016
  • QA review of released products

Antigen Details

Structure
C-type lectin, type II transmembrane glycoprotein, covalently associates with NKG2, 43 kD
Distribution

NK cells , T subset

Function
Inhibits NK function
Cell Type
NK cells, T cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Lopez-Botet M, et al. 1997. Immunol. Rev. 155:165.
2. Moretta A, et al. 1997. Immunol. Rev. 155:105.

Gene ID
3824 View all products for this Gene ID
UniProt
View information about CD94 on UniProt.org
Go To Top Version: 1    Revision Date: 09.09.2024

For Research Use Only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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