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GMP PE anti-human CD62P (P-Selectin) Antibody

Pricing & Availability
Clone
AK4 (See other available formats)
Workshop
VI P-44
Other Names
GMP-140, PADGEM
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
AK4_GMP_PE_CD62P_Antibody_111523
Typical results from thrombin-activated human peripheral blood platelets stained either with AK4 PE used at 5 µL/test (filled histogram) or with an isotype control (open histogram).
  • AK4_GMP_PE_CD62P_Antibody_111523
    Typical results from thrombin-activated human peripheral blood platelets stained either with AK4 PE used at 5 µL/test (filled histogram) or with an isotype control (open histogram).
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260364 100 tests 305 CHF
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Description

CD62P is a 140 kD type I transmembrane glycoprotein also known as P-selectin, platelet activation-dependent granule membrane protein (PADGEM), and GMP-140. It is expressed on activated platelets, megakaryocytes, and endothelial cells. CD62P is primarily stored in secretory α-granules in platelets and Weibel-Palade bodies in endothelial cells, and is rapidly relocated to the plasma membrane upon activation. The ligands for CD62P are CD162 and CD24. A primary function of CD62P is cell adhesion during neutrophil rolling, and platelet-neutrophil and platelet-monocyte interactions.

Product Details
Quality Statement This BioLegend’s GMP-RUO reagent is a fluorochrome-labeled monoclonal antibody single reagent for Research Use Only. Suitable for use in laboratory-developed multicolor flow cytometry tests. Single GMP-RUO products can be used in various multicolor flow cytometry combinations. Laboratory need to establish the GMP-RUO performance characteristics in combination with other reagents in normal and pathological samples.
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA) and a stabilizer
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
100 µg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections4 and in vitro blocking of adhesion of platelets1. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 304947 & 304948).

Application References

(PubMed link indicates BioLegend citation)
  1. Skinner M, et al. 1991. J. Biol. Chem. 266:5371. (Block)
  2. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
  3. Yen YT, et al. 2006. J. Virol. 80:2684.
  4. Sato Y, et al. 2005. Blood 106:428. (IHC)
Disclaimer

GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include:

  • Batch-to-batch consistency
  • Material traceability
  • Documented procedures
  • Documented employee training
  • Equipment maintenance and monitoring records
  • Lot-specific certificates of analysis
  • Quality audits per ISO 13485:2016
  • QA review of released products

Antigen Details

Structure
Selectin, type I glycoprotein, 140 kD
Distribution

Activated platelets, megakaryocytes, endothelial cells

Function
Adhesion, neutrophil rolling, platelet-neutrophil and platelet-monocyte interactions
Ligand/Receptor
CD162 (PSGL-1), CD24 and sialylated Lewis X
Cell Type
Endothelial cells, Megakaryocytes, Neutrophils, Platelets
Biology Area
Cell Adhesion, Cell Biology, Immunology, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. McEver R, et al. 1995. J. Biol. Chem. 270:11025.
2. Varki A. 1994. P. Natl. Acad. Sci. USA 91:7390.

Gene ID
6403 View all products for this Gene ID
UniProt
View information about CD62P on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.

Other Formats

View All CD62P Reagents Request Custom Conjugation
Description Clone Applications
APC anti-human CD62P (P-Selectin) AK4 FC
FITC anti-human CD62P (P-Selectin) AK4 FC
PE anti-human CD62P (P-Selectin) AK4 FC
PE/Cyanine5 anti-human CD62P (P-Selectin) AK4 FC
Purified anti-human CD62P (P-Selectin) AK4 FC,IHC-F
Biotin anti-human CD62P (P-Selectin) AK4 FC
Alexa Fluor® 488 anti-human CD62P (P-Selectin) AK4 FC
Alexa Fluor® 647 anti-human CD62P (P-Selectin) AK4 FC
Brilliant Violet 605™ anti-human CD62P (P-Selectin) AK4 FC
Brilliant Violet 421™ anti-human CD62P (P-Selectin) AK4 FC
PE/Cyanine7 anti-human CD62P (P-Selectin) AK4 FC
PerCP/Cyanine5.5 anti-human CD62P (P-Selectin) AK4 FC
Alexa Fluor® 700 anti-human CD62P (P-Selectin) AK4 FC
PE/Dazzle™ 594 anti-human CD62P (P-Selectin) AK4 FC
APC/Fire™ 750 anti-human CD62P (P-Selectin) AK4 FC
TotalSeq™-A0218 anti-human CD62P (P-Selectin) AK4 PG
Brilliant Violet 650™ anti-human CD62P (P-Selectin) AK4 FC
Brilliant Violet 510™ anti-human CD62P (P-Selectin) AK4 FC
Brilliant Violet 711™ anti-human CD62P (P-Selectin) AK4 FC
APC/Cyanine7 anti-human CD62P (P-Selectin) AK4 FC
Brilliant Violet 785™ anti-human CD62P (P-Selectin) AK4 FC
TotalSeq™-C0218 anti-human CD62P (P-Selectin) AK4 PG
Ultra-LEAF™ Purified anti-human CD62P (P-Selectin) AK4 FC
TotalSeq™-B0218 anti-human CD62P (P-Selectin) Antibody AK4 PG
TotalSeq™-D0218 anti-human CD62P (P-Selectin) AK4 PG
PE anti-human CD62P AK4 FC
FITC anti-human CD62P AK4 FC
GMP FITC anti-human CD62P (P-Selectin) AK4 FC
GMP PE anti-human CD62P (P-Selectin) AK4 FC
Go To Top Version: 1    Revision Date: 12.13.2023

For Research Use Only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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