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GoInVivo™ Purified anti-mouse CD152 Antibody

Pricing & Availability
Clone
9H10 (See other available formats)
Regulatory Status
RUO
Other Names
Cytotoxic T Lymphocyte-Associated Antigen-4 (CTLA-4), Ly-56
Isotype
Syrian Hamster IgG
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Product Citations
publications
9H10_GoInVivo_CD152_Antibody_080916
Anti-mouse CD152 (CTLA-4) inhibits production of IL-2 by Jurkat cells. When Jurkat cells are stimulated with PHA (2.5 µg/ml) and soluble CD80 (B7.1, 500 ng/ml), the addition of soluble mouse CTLA-4 competes with CD80 and inhibits the production of IL-2 in a dose dependent manner (diamonds). When a fixed concentration of CTLA-4 is selected and pre-incubated with increasing concentrations of anti-mouse CTLA-4 (clone 9H10), the production of IL-2 is restored as the antibody blocks the activity of CTLA-4, but not the isotype control (clone SHG-1).
  • 9H10_GoInVivo_CD152_Antibody_080916
    Anti-mouse CD152 (CTLA-4) inhibits production of IL-2 by Jurkat cells. When Jurkat cells are stimulated with PHA (2.5 µg/ml) and soluble CD80 (B7.1, 500 ng/ml), the addition of soluble mouse CTLA-4 competes with CD80 and inhibits the production of IL-2 in a dose dependent manner (diamonds). When a fixed concentration of CTLA-4 is selected and pre-incubated with increasing concentrations of anti-mouse CTLA-4 (clone 9H10), the production of IL-2 is restored as the antibody blocks the activity of CTLA-4, but not the isotype control (clone SHG-1).
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106209 500 mg 20735 CHF
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106207 50 mg 3575 CHF
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106208 100 mg 5990 CHF
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106206 25 mg 2365 CHF
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106210 1 g 32175 CHF
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106205 5 mg 770 CHF
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Description

CD152 is a 33 kD member of the immunoglobulin superfamily also known as CTLA-4 or Ly-56. It is expressed on activated T and B lymphocytes. CD152 is similar to CD28 in amino acid sequence, structure, and genomic organization and these two receptors share common B7 family counter-receptors (B7-1, B7-2). Whereas CD28 delivers a costimulatory signal in T cell activation, CTLA-4 negatively regulates cell-mediated immune responses. CD152 is thought to play a role in the induction and maintenance of immunological tolerance, as well as the development of protective immunity and thymocyte regulation. The 9H10 antibody can enhance T cell co-stimulation by blocking CTLA-4 interactions with the B7 co-receptors, favoring CD28 interactions.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Syrian Hamster
Immunogen
Mouse CTLA-4-human IgG1 fusion protein
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 1.0 EU/mg of the protein (< 0.1 pg/µg of the protein) as determined by the LAL test.
Preparation
The GoInVivo™ antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This GoInVivo™ solution contains no preservative; handle under aseptic conditions.
Application

ELISA - Quality tested
Block, Costim, FC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

For in vivo and in vitro applications, we recommend to perform a pilot experiment to determine the optimal concentration to use for each particular experiment.

Application Notes

GoInVivo™ products are guaranteed to be pathogen-free based on the IDEXX BioResearch IMPACT test via PCR. For a full listing of pathogens tested, visit the GoInVivo™ Webpage.

Additional reported applications (for the relevant formats) include: in vivo and in vitro blocking1-4 of CTLA-4 action by soluble antibody and promotion of in vitro CTLA-4 signalling by immobilized antibody1. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302), is recommended prior to immunofluorescent staining. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-Syrian hamster IgG (Cat. No. 405601) second step, followed by SAv-PE (Cat. No. 405204)).

Application References

(PubMed link indicates BioLegend citation)
  1. Krummel MF, et al. 1995. J. Exp. Med. 182:459. (Block, Costim, FC)
  2. Krummel MF, et al. 1996. Int. Immunol. 8:519. (Block)
  3. Leach DR, et al. 1996. Science 271:1734. (Block)
  4. Wagner Jr, DH, et al. 1996. J. Exp. Med. 184:1631. (Block)
Product Citations
  1. Wang Y, et al. 2016. Exp Ther Med. 81:12. PubMed
  2. Zhu Y, et al. 2023. Sci Immunol. eade4656:8. PubMed
RRID
AB_2616629 (BioLegend Cat. No. 106209)
AB_2616629 (BioLegend Cat. No. 106207)
AB_2616629 (BioLegend Cat. No. 106208)
AB_2616629 (BioLegend Cat. No. 106206)
AB_2616629 (BioLegend Cat. No. 106210)
AB_2616629 (BioLegend Cat. No. 106205)
Disclaimer

This product may be used for research purposes only. It is not licensed for resale and may only be used by the buyer. This product may not be used and is not licensed for clinical assays where the results of such assays are provided as a diagnostic service. If a diagnostic or therapeutic use is anticipated then a license must be requested from the University of California. The availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California.

Antigen Details

Structure
Ig superfamily, 33 kD
Distribution

Activated T cells

Function
Negative regulator of T cell activation
Ligand/Receptor
CD80 (B7-1), CD86 (B7-2)
Cell Type
T cells, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Allison JP, et al. 1995. Science 270:932.
3. Waterhouse P, et al. 1995. Science 270:985.
4. Linsley PS, et al. 1991. J. Exp. Med. 174:561.

Gene ID
12477 View all products for this Gene ID
UniProt
View information about CD152 on UniProt.org

Other Formats

View All CD152 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse CD152 9H10 ELISA,FC,Block,Costim
GoInVivo™ Purified anti-mouse CD152 9H10 ELISA,FC,Block,Costim
Ultra-LEAF™ Purified anti-mouse CD152 9H10 ELISA,FC,Block,Costim
Go To Top Version: 5    Revision Date: 11.30.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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