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GoInVivo™ Purified anti-mouse CD223 (LAG-3) Antibody

Pricing & Availability
Clone
C9B7W (See other available formats)
Regulatory Status
RUO
Other Names
LAG3, LAG-3
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
C9B7W_GoInVivoPurified_CD223_LAG-3_Antibody_FC_122215
Anti-mouse LAG-3, or CD223, inhibits the binding of mouse LAG-3 to Galectin-3. Mouse LAG-3 binds immobilized mouse Galectin-3 in a dose dependent manner (purple circles). Mouse LAG-3 (1 µg/mL) was pre-incubated with increasing concentrations of anti-mouse LAG-3 (clone C9B7W, purple squares) prior to adding to wells containing immobilized mouse Galectin-3. Clone C9B7W inhibits the interaction between LAG-3 and Galectin-3 in a dose dependent manner.
  • C9B7W_GoInVivoPurified_CD223_LAG-3_Antibody_FC_122215
    Anti-mouse LAG-3, or CD223, inhibits the binding of mouse LAG-3 to Galectin-3. Mouse LAG-3 binds immobilized mouse Galectin-3 in a dose dependent manner (purple circles). Mouse LAG-3 (1 µg/mL) was pre-incubated with increasing concentrations of anti-mouse LAG-3 (clone C9B7W, purple squares) prior to adding to wells containing immobilized mouse Galectin-3. Clone C9B7W inhibits the interaction between LAG-3 and Galectin-3 in a dose dependent manner.
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125214 500 mg 18425 CHF
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125213 100 mg 5335 CHF
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125215 1 g 28600 CHF
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125217 25 mg 2365 CHF
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125218 50 mg 3575 CHF
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125216 5 mg 770 CHF
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Description

CD223, also known as LAG-3, is 70 kD type I transmembrane glycoprotein within the Ig superfamily. It is the gene product of lymphocyte activation gene-3 (Lag3) with structural similarity to CD4. CD223 is expressed on activated T cells and NK cells, as well as T regulatory cells. CD223, like CD4, binds MHC class II molecules, but with higher affinity than CD4. In association with the CD3 complex, CD223 has been shown as a negative regulatory molecule to inhibit TCR-mediated signaling and regulate homeostatic T cell expansion. In contrast to inhibition, CD223 has an activation function to promote immune responses by inducing MHC class II signaling in antigen presenting cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Murine CD223-Ig fusion protein
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 1.0 EU/mg of the protein (< 0.1 pg/µg of the protein) as determined by the LAL test.
Preparation
The GoInVivo™ antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This GoInVivo™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
FA, IP, ELISA - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

For in vivo and in vitro applications, we recommend to perform a pilot experiment to determine the optimal concentration to use for each particular experiment.

Application Notes

GoInVivo™ products are guaranteed to be pathogen-free based on the IDEXX BioResearch IMPACT test via PCR. For a full listing of pathogens tested, visit the GoInVivo™ Webpage.

Application References

(PubMed link indicates BioLegend citation)
  1. Workman CJ, et al. 2002. Eur. Immunol. 32:2255. (IP, ELISA)
  2. Workman CJ, et al. 2005. J. Immunol. 174:688. (FC)
  3. Li N, et al. 2004. J. Immunol. 173:6806. (IP, ELISA)
RRID
AB_2566284 (BioLegend Cat. No. 125214)
AB_2566284 (BioLegend Cat. No. 125213)
AB_2566284 (BioLegend Cat. No. 125215)
AB_2566284 (BioLegend Cat. No. 125217)
AB_2566284 (BioLegend Cat. No. 125218)
AB_2566284 (BioLegend Cat. No. 125216)

Antigen Details

Structure
Ig superfamily
Distribution

T cells and NK cells, as well as T regulatory cells

Cell Type
NK cells, T cells, Tregs
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Baixeras E et al. 1992. J. Exp. Med. 176:327.

Gene ID
16768 View all products for this Gene ID
UniProt
View information about CD223 on UniProt.org
Go To Top Version: 5    Revision Date: 11.30.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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