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Golgi Detection Probe Red

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Regulatory Status
RUO
Other Names
TMR Ceramide Golgi Probe
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A_Golgi_Detection_Probe_Red_080123
Overlay of live-cell fluorescence imaging of HeLa cells stained with Golgi Detection Probe Red (red). Nuclei were stained with Hoechst 33342 (blue). Images were captured using a 40X objective. Scale bar: 20 µm
  • A_Golgi_Detection_Probe_Red_080123
    Overlay of live-cell fluorescence imaging of HeLa cells stained with Golgi Detection Probe Red (red). Nuclei were stained with Hoechst 33342 (blue). Images were captured using a 40X objective. Scale bar: 20 µm
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Description

The Golgi apparatus is a complex of vesicles and folded membranes within the cytoplasm of eukaryotic cells. The Golgi apparatus is involved in protein and lipid secretion and intracellular transport. It modifies proteins and lipids that have been built in the endoplasmic reticulum (ER) and prepares them for export outside of the cell. It also plays a significant role in the formation of lysosomes. Golgi Detection Probe Red can selectively bind to the Golgi apparatus and provides a simple and rapid way to perform live-cell fluorescent imaging.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Mouse, Rat
Formulation
1 vial of Golgi Detection Probe, 1 vial of DMSO
Preparation
Please refer to detailed protocol in application notes.
Storage & Handling
-20°C
Application

Live cell imaging - Quality tested
Recommended Usage

Please refer to detailed protocol in application notes.

Application Notes

The Golgi Detection Probe Red is a fluorescently labeled ceramide, which selectively binds to the Golgi apparatus and provides a simple method to examine Golgi morphology in live cells. Using the recommended volume of 100 μL per well in a 96-well plate, this product supplies enough probe for 100 tests.
 

Component:
Lyophilized Golgi Detection Probe, 1 vial
DMSO, 1 vial

Required Materials Not Included:
Phenol-free media or HBSS

Imaging Guidelines:
Ex/Em = 544/570 nm 
Fluorescence microscope filter set: Cyanine3/TRITC filter

Flow Guidelines:
Analysis in Cyanine3 or similar channel

Assay Protocol:
1. Prepare the 100X Golgi Detection Probe Stock Solution by adding 100 µL of DMSO to the lyophilized probe. Gently mix until dissolved.
Note: Store the unused Golgi Detection Probe Stock Solution at -20°C in single use aliquots to avoid freeze thaw cycles.
2. Allow the 100X Golgi Detection Probe Stock Solution to reach room temperature.
3. Prepare Golgi Detection Probe Working Solution by diluting 1:100 in Phenol-free media or HBSS
Note: Staining conditions and probe concentration may have to be modified according to cell type and assay used.
4. Remove growth media from cells and add the Golgi Detection Probe Working Solution.
Note: We recommend 100 µL per well for a 96-well plate.
5. Incubate cells at 37°C for 20-30 minutes.
6. Wash cells twice with phenol free media or HBSS and then add desired volume of phenol-free media or HBSS.
7. Proceed immediately to live-cell imaging.

Antigen Details

Distribution

Golgi

Biology Area
Cell Biology, Cell Structure, Protein Trafficking and Clearance
Molecular Family
Golgi Markers
Gene ID
NA
Go To Top Version: 1    Revision Date: 08.01.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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