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HA Peptide Tag (Previously Covance catalog# PEP-101P)

Pricing & Availability
Regulatory Status
RUO
Other Names
HA epitope tag, HA1, HA2, hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain, YPYDVPDYA, Hemagglutinin tag
Previously
Covance Catalog# PEP-101P
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Product Citations
publications
Purified_HA-Pep_Antibody_ELISA_032615
Direct ELISA showing reactivity of HA.11 monoclonal antibody to HA.11 peptide, coated on the plate at 2ug/mL.
  • Purified_HA-Pep_Antibody_ELISA_032615
    Direct ELISA showing reactivity of HA.11 monoclonal antibody to HA.11 peptide, coated on the plate at 2ug/mL.
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931401 1 mg 213 CHF
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Description

Influenza hemagglutinin (HA) is a viral membrane glycoprotein responsible for binding of the virus to the cells expressing sialic acid-containing surface receptors, such as erythrocytes and the cells in the upper respiratory tract. The hemagglutinin epitope, YPYDVPDYA, has been widely used as a tag in recombinant protein expression vectors.

Product Details
Technical Data Sheet (pdf)

Product Details

Purity
>90% pure by HPLC
Formulation
Lyophilized powder.
Preparation
Lyophilized (trace TFA salts).
Storage & Handling
Store at -20°C or below. Do not store in frost-free freezers.
Application

ELISA, IP, WB
Recommended Usage

Each lot of this product is quality control tested by ELISA.

Soluble in PBS. The optimal working dilution should be determined for each specific assay condition.
• Elution of epitope tag fusion proteins from immunoaffinity columns: 400 ug/mL
• Competitive inhibition of antibody in western or immunostaining: 10-fold molar excess over optimized (low) antibody

Application Notes

This synthetic peptide (H2N-CYPYDVPDYA-COOH) corresponds to the epitope detected by the anti-HA HA.11 monoclonal antibody.

This peptide can be used in competition experiments, or to elute bound HA-fusion proteins from the HA.11 affinity matrix (Cat. No. 900801).

Application References

(PubMed link indicates BioLegend citation)
  1. Kizhatil K, et al. 2002. J. Neurosci. 22: 7948.
  2. Ferrando A, et al. 2001. Nucleic Acids Res. 29:3685.

Antigen Details

Biology Area
Cell Biology
Gene ID
NA
UniProt
View information about HA Tag on UniProt.org
Go To Top Version: 2    Revision Date: 07.27.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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