HRP anti-Clathrin Heavy Chain (CLTCL2) Antibody anti-CLTCL2

Pricing & Availability

Clone: TD.1 (See other available formats)
Regulatory Status: RUO
Other Names: Clathrin heavy chain 1, clathrin, heavy polypeptide-like 2, clathrin heavy chain on chromosome 17
Isotype: Mouse IgG1, κ
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Product Citations: publications

TD-1_HRP_Clathrin_Heavy_Chain_Antibody_WB_082718 — Western blot of HRP anti-Clathrin Heavy Chain (CLTCL2) antibody (clone TD.1). Lane 1: Molecular weight marker; Lane 2: 20 µg of human cerebellum lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.

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813904

100 µg

323 CHF

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Description

Clathrin consists of three heavy chains (192 kD) and three light chains (LCa or LCb, 25-29 kD) and self assembles into a polyhedral cytoskeletal element. The self assembly facilitates clathrin's central role in membrane sorting and selective transport within the cell.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human, Drosophila
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: This monoclonal antibody was raised using the globular N-terminal domain of clathrin heavy chain.
Formulation: This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation: The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody reacts with clathrin heavy chains (CHC). The antibody is useful for immunoblotting of clathrin heavy chains from cow CHC and immunofluorescence. It does not react with native clathrin in immunoprecipitation.

Reactivity to Drosophila was only verified with the purified format.
RRID: AB_2750414 (BioLegend Cat. No. 813904)

Antigen Details

Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Gene ID: 1213 View all products for this Gene ID
UniProt: View information about Clathrin Heavy Chain on UniProt.org

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Western Blotting Protocol

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Other Formats

View All Clathrin Heavy Chain (CLTC) Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-Clathrin Heavy Chain (CLTCL2)	TD.1	WB,ICC
HRP anti-Clathrin Heavy Chain (CLTCL2)	TD.1	WB

Certificate of Analysis

Go To Top Version: 2 Revision Date: 03.12.2021

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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