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HRP anti-Tubulin β 3 (TUBB3) Antibody

Pricing & Availability
Clone
TUJ1 (See other available formats)
Regulatory Status
RUO
Other Names
CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, TUBB4, Tubulin beta-3 chain, tubulin beta-III, tubulin beta-4 chain, class III beta-tubulin
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
B_TUJ1_HRP_Tubulin-beta-3-TUBB3_2_WB_071217
Western blot of HRP anti-Tubulin β 3 (TUBB3) antibody (clone TUJ1). Lane 1: 5 µg of human brain tissue lysate; Lane 2: 5 µg of rat brain tissue lysate; Lane 3: 5 µg of mouse brain tissue lysate. The blots were incubated with the HRP-conjugated or mouse IgG2a antibody at 1 µg/mL overnight at 4°C. HRP Tubulin β 3 was visualized using chemiluminescence detection. HRP labeled goat anti-mouse secondary antibody was used for the blot that was incubated with the IgG2a primary antibody followed by chemiluminescence detection.
  • B_TUJ1_HRP_Tubulin-beta-3-TUBB3_2_WB_071217
    Western blot of HRP anti-Tubulin β 3 (TUBB3) antibody (clone TUJ1). Lane 1: 5 µg of human brain tissue lysate; Lane 2: 5 µg of rat brain tissue lysate; Lane 3: 5 µg of mouse brain tissue lysate. The blots were incubated with the HRP-conjugated or mouse IgG2a antibody at 1 µg/mL overnight at 4°C. HRP Tubulin β 3 was visualized using chemiluminescence detection. HRP labeled goat anti-mouse secondary antibody was used for the blot that was incubated with the IgG2a primary antibody followed by chemiluminescence detection.
  • C_TUJ1_HRP_Tubulin-beta-3-TUBB3_2_IHC-P_071217
    IHC staining of HRP anti-Tubulin β 3 (TUBB3) antibody (clone TUJ1) on formalin-fixed paraffin-embedded human cerebellum brain tissue. After antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with the primary antibody at 2.5 µg/mL for one hour at room temperature. DAB was used for detection followed by hematoxylin counterstaining and bluing solution counterstaining, according to the protocol provided.
  • D_TUJ1_HRP_Tubulin-beta-3-TUBB3_2_IHCP_071217
    IHC staining of anti-Tubulin β 3 (TUBB3) antibody (clone TUJ1) on formalin-fixed, paraffin-embedded human cerebellum tissue. Following antigen retrieval using Sodium Citrate H.E.I.R, the tissue was incubated with HRP conjugated antibody at room temperature for one hour at 1 µg/mL. Biolegend's Ultra-Streptavidin HRP was used for detection for mouse IgG2a antibody. Tissues were counterstained with hematoxylin.
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801205 25 µg 136 CHF
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801206 100 µg 335 CHF
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Description

Tubulin is the main component of microtubules. In adults, tubulin beta 3 (TUBB3) is primarily expressed in neurons and is commonly used as a neuronal marker. It plays an important role in neuronal cell proliferation and differentiation. Mutations in this gene cause congenital fibrosis of the type 3 extraocular muscles. Tubulin beta 3 (TUBB3) is also found in a wide range of tumors. Studies indicate that it is a predictive and prognostic marker in various tumors.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was raised against microtubules derived from rat brain.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per ml. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: flow cytometry4, immunofluorescence microscopy1-5,7, immunohistochemistry5,7, Western blotting8, and spatial biology (IBEX)9,10.

This antibody is well characterized and highly reactive to neuron specific Class III ß-tubulin (ßIII). TUJ1 does not identify ß-tubulin found in glial cells. TUJ1 recognizes an epitope located within the last 15 C-terminal residues8.

Application References

(PubMed link indicates BioLegend citation)
  1. Nishimura K, et al. 2017. PLoS One. 12(1): e0170568. (ICC)
  2. Jongbloets J, et al. 2017. Nat Commun. 8: 14666. (ICC) PubMed
  3. Liu W.J, et al. 2015. Eur J Histochem. 59(1): 2464. (ICC) PubMed
  4. Chintalapudi SR, et al. 2016. Front Aging Neursci. 8:93. (FC, ICC) PubMed
  5. Ambasudhan R, et al. 2011. Cell Stem Cell. 9(2):113. (IHC, ICC)
  6. Hu X., et al. 2006. Nature Neuroscicene. 9(12):1520. (WB) PubMed
  7. Zechner D., et al. 2003. Develop Biology. 258(2):406. (ICC, IHC)
  8. Lee MK, et al. 1990. Proc. Natl. Acad. Sci. USA 18:7195. (WB)
  9. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  10. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
RRID
AB_2721333 (BioLegend Cat. No. 801205)
AB_2721333 (BioLegend Cat. No. 801206)
Disclaimer

Covered by US patents 5,675,063 and 7,429,487. Sold under license from Epitomics.

Antigen Details

Structure
Tubulin β 3 is a 450 amino acid protein with a molecular mass of ~50 kD.
Distribution

Tissue distribution: central and peripheral nervous system.
Cellular distribution: cytosol, cytoskeleton and nucleus.

Function
Tubulin β 3 is the major constituent of microtubules, and plays a critical role in proper axon guidance and maintenance.
Interaction
Alpha tubulin, kinesin and dynein.
Cell Type
Mature Neurons, Neural Stem Cells
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Microtubules
Antigen References

1. Zhao X, et al. 2017. Med Sci Monit. 22: 3915.
2. Lebok P, et al. 2016. Oncol Lett. 11(3):1987.
3. Du J, et al. 2015. BMC Cancer. 15:536. PubMed
4. Rogue DM., et al. 2013. Clin Exp Metastasis. 31(1): 101.
5. Ploussard G, et al. 2010. Cancer Res. 70(22):9253. PubMed

Gene ID
10381 View all products for this Gene ID
UniProt
View information about Tubulin beta-3 on UniProt.org
Go To Top Version: 3    Revision Date: 01.16.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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