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HRP Avidin

Pricing & Availability
Regulatory Status
RUO
Other Names
Avidin-Peroxidase, Avidin-Horseradish Peroxidase, Av-HRP
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Product Citations
publications
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405103 1 mL 129 CHF
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Description

Avidin binds to biotin with high affinity. Avidin-HRP is useful for detecting biotinylated antibodies, e.g., in ELISA or Western blotting. For ELISA applications, HRP will act on soluble substrates, such as ABTS or TMB, to yield a colorimetric reaction. For Western blotting or ELISPOT applications, HRP will act on precipitating substrates, such as 4CN, to yield a colorimetric reaction.

Product Details
Technical Data Sheet (pdf)

Product Details

Formulation
Buffered solution containing bovine protein and preservatives (methylisothiazolone, bromonitrodioxane, and other active isothiazolones).
Preparation
Avidin is conjugated with horseradish peroxidase under optimal conditions.
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Avoid exposure to sodium azide.
Application

ELISA - Quality tested
ELISPOT, IHC, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA or Western blot analysis, the reagent should be titrated between 1:1000 - 1:5000 to determine optimal conditions. For ELISPOT, dilute at 1:500 - 1:2500 to determine optimal conditions. Avoid using biotin-containing solutions as diluents and solutions containing sodium azide. Sodium azide is an inhibitor of horseradish peroxidase. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Avidin-HRP is useful as a second step reagent for indirect enzymatic labelling, in conjunction with biotinylated primary antibodies. Av-HRP is recommended for ELISA, ELISPOT and Western blotting, when used with the relevant substrate system.

Application References

(PubMed link indicates BioLegend citation)
  1. Qiu LQ, et al. 2012. J Immunol. 188:5150. PubMed
  2. McLaughlin HP, et al. 2013. J Med Microbiol. 62:185. PubMed
  3. Chan WL, et al. 2013. Nucleic Acids Res. 41:3734. PubMed
  4. Macauley MS, et al. 2014. J Biol Chem. 289:351489. PubMed
  5. Roncolato EC, et al. 2015. Infect Immun. 83:713. PubMed
Product Citations
  1. Altemus J, et al. 2022. J Cell Physiol. 237:3001. PubMed
  2. Vazquez-Lombardi R, et al. 2022. Immunity. 55:1953. PubMed
  3. Matsuzawa M, et al. 2023. Nat Commun. 14:1417. PubMed
  4. Bracho-Sanchez E, et al. 2023. Nat Biomed Eng. . PubMed
  5. Soe PP, et al. 2023. Int J Mol Sci. 24:. PubMed
  6. Clausen TM, et al. 2020. Cell. 183:1043. PubMed
  7. Montégut L, et al. 2022. STAR Protoc. 3:101095. PubMed
  8. Yuan S, et al. 2021. Nature. 593:418. PubMed
  9. Clausen TM, et al. 2020. bioRxiv. . PubMed
  10. Roncolato E, et al. 2015. Infect Immun . 83:713. PubMed
  11. Qiu L, et al. 2012. J Immunol. 188:5150. PubMed
  12. Wu J, et al. 2020. Immunity. 53:115. PubMed
  13. McLaughlin H, et al. 2013. J Med Microbiol. 62:185. PubMed
  14. Chan W, et al. 2013. Nucleic Acids Res. 41:3734. PubMed
  15. Tada R, et al. 2015. PLoS One. 10: e0139785. PubMed
  16. Li Y, Kaneda T 2016. Sci Rep. 6: 25077. PubMed
  17. Macauley M, et al. 2014. J Biol Chem. 289:35149. PubMed
  18. Kojima H, et al. 2021. Scand J Immunol. 93:e13020. PubMed
  19. Narayanasamy A, et al. 2011. J Proteomics. 74:2948. PubMed
  20. Heinzel C, et al. 2021. Front Immunol. 12:753435. PubMed
  21. Salehi S, et al. 2017. PLoS One. 10.1371/journal.pone.0163614. PubMed
  22. Yabe R, et al. 2021. Nat Commun. 0.565277778. PubMed
  23. Tan EE, et al. 2020. J Clin Invest. 130:5817. PubMed

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Go To Top Version: 3    Revision Date: 01.31.2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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