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ELISA MAX™ Deluxe Set Human CXCL10 (IP-10)

Pricing & Availability
Regulatory Status
RUO
Other Names
Interferon gamma-inducible protein 10 (IP10), Interferon gamma induced factor, gIP-10, IFI10, INP10, CXCL10 Precoated ELISA Kit
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Product Citations
publications
CXCL10_Human_ELISA_Kit_Deluxe_041113
  • CXCL10_Human_ELISA_Kit_Deluxe_041113
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439904 5 plates 347 CHF
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Description

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CXCL10, also known as IP-10, is a glutamic acid-leucine-arginine motif negative chemokine structurally and functionally related to MIG (CXCL9) and ITAC (CXCL11). CXCL10 is induced in a variety of cells in response to interferon-γ and lipopolysaccharide. These cell types include monocytes, macrophages, fibroblasts, and epithelial cells. CXCL10 (IP-10) chemoattracts CD4+ and CD8+ T cells, NK, and NKT cells via binding to its receptor CXCR3, which is shared with MIG and ITAC.

CXCL10 inhibits neovascularization in tumors and in wound healing in vivo. It also has anti-proliferative effects on endothelial cells in vitro, and angiostatic and antitumor effects in vivo. It has been suggested that the antiproliferative effect of CXCL10 in endothelial cells is CXCR3-independent and that it is mediated through glycosaminoglycan interaction. CXCL10 also possesses antimicrobial activity against E. coli and L. monocytogenes, and both the spore and bacillus forms of B. anthracis. It has been reported that CXCL10 may be important in the pathogenesis of chronic HCV infection, and the circulating CXCL10 concentration was increased in the early and subclinical stage of Type I diabetes patients. CXCL10 was significantly up-regulated in the salivary glands of patients with Sjögren's Syndrome. It also has been reported that CXCL10 was increased in the urine of patients with pulmonary diseases in the absence of renal dysfunction.

Both the ELISA MAX™ Set Standard and ELISA MAX™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Human CXCL10/IP-10 ELISA MAX™ Capture Antibody (200X)
  • Human CXCL10/IP-10 ELISA MAX™ Detection Antibody (200X)
  • Human CXCL10/IP-10 Standard
  • Avidin-HRP (1000X)
  • Substrate Solution F
  • Matrix Diluent C (for serum and plasma samples)
  • Coating Buffer A (5X)
  • Assay Diluent A (5X)

Product Details

Verified Reactivity
Human
Application

ELISA
 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Application References

(PubMed link indicates BioLegend citation)
  1. Abou El Hassan M, et al. 2015. PLoS One.10:126466. PubMed
Product Citations
  1. Fionda C, et al. 2022. Front Immunol. 13:1039120. PubMed
  2. Di Nitto C, et al. 2023. Pharmaceutics. 15:. PubMed
  3. Hassan M, et al. 2015. PLoS One. 10:126466. PubMed
  4. AbuSamra DB, et al. 2021. Cells. 10:. PubMed
  5. Henrich IC, et al. 2021. Cancer Res. 81:2171. PubMed
  6. Read SA, et al. 2021. J Inflamm Res. 14:1257. PubMed
  7. Millrine D, et al. 2016. Proc Natl Acad Sci U S A. 113: 10625 - 10630. PubMed
  8. Luteijn RD, et al. 2019. Nature. 573:434. PubMed
  9. Congy-Jolivet N, et al. 2022. EBioMedicine. 80:104047. PubMed
  10. Huang YC, et al. 2021. Toxins (Basel). 13:. PubMed
Sensitivity
4 pg/mL
Standard Range
15.6-1,000 pg/mL
Materials Not Included
  • Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
  • Deionized (DI) water
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 µm filtered.
  • Wash Buffer (Phosphate-Buffered Saline (PBS) + 0.05% Tween-20, pH=7.4). BioLegend Cat. No. 421601 is recommended.
  • Wash bottle or automated microplate washer
  • Stop Solution (e.g. 2N H2SO4) Biolegend Cat. No. 423001 is recommended
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Sealer
  • Absorbent paper

Antigen Details

Cell Sources
Monocytes, macrophages, fibroblasts, and epithelial cells
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Cytokines/Chemokines, Growth Factors
Gene ID
3627 View all products for this Gene ID
UniProt
View information about CXCL10 on UniProt.org

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 4    Revision Date: 09.12.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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