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ELISA MAX™ Deluxe Set Human IL-1β

Pricing & Availability
Regulatory Status
RUO
Other Names
IL-1b Elisa Kit
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Product Citations
publications
Human_IL-1beta_012011
  • Human_IL-1beta_012011
Cat # Size Price Quantity Check Availability Save
437004 5 plates 347 CHF
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437015 10 Plates 586 CHF
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437016 20 Plates 938 CHF
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Description

ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation

BioLegend's ELISA Max™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price!

BioLegend's ELISA Max™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.

Both the ELISA Max™ Set Standard and ELISA Max™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.

ELISA Max™ Set Deluxe includes pre-titrated capture antibody, pre-titrated biotinylated detection antibody, pre-titrated Avidin-HRP, conveniently-sized assay calibration standards, easy-to-follow instructions, as well as, assay diluent, coating buffer,  and TMB substrate. Complete and ready to use!

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Human IL-1β ELISA MAX™ Capture Antibody (200X)
  • Human IL-1β ELISA MAX™ Detection Antibody (200X)
  • Human IL-1β Standard
  • Avidin-HRP (1000X)
  • Substrate Solution F
  • Coating Buffer A (5X)
  • Assay Diluent A (5X)
  • Assay Buffer D
  • Blocking Buffer A (2X)

*Earlier lots of 437004, 437015, 437016 did not include Blocking Buffer A (2X) reagent, and the Assay Diluent A (1X) should be used for blocking non-specific binding of the assay. The other steps in the protocol should be performed with no changes. The correct list of the supplied reagents and the corresponding protocol can be found in the lot-specific CoA.

Product Details

Verified Reactivity
Human
Application

ELISA
 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Application Notes

The assay kits will no longer include ELISA microwell plates. Recommended microwell plates can be purchased separately under Cat. No. 423501. Please contact your BioLegend sales representative for bulk pricing quotations.

BioLegend's mouse and human IL-1β ELISA kits use antibodies raised against the mature form of IL-1β. However, because pro-IL-1β contains the mature form, these antibodies likely recognize both the pro- and the mature forms of the protein. The human IL-1β ELISA kits have been shown to equally recognize both pro- and mature forms of IL-1β.

Product Citations
  1. Ghait M, et al. 2022. Eur J Immunol. 52:907. PubMed
  2. Hsu CG, et al. 2022. Cell Death Differ. 29:1790. PubMed
  3. Fuchs T, et al. 2016. Immunol Lett. 173:21-25. PubMed
  4. Yndart Arias A, et al. 2023. Sci Rep. 13:7376. PubMed
  5. Marín-Palma D, et al. 2023. Front Immunol. 14:1161135. PubMed
  6. Kobpornchai P, et al. 2022. Front Cell Infect Microbiol. 12:919835. PubMed
  7. Zhou J, et al. 2021. Front Immunol. 12:656242. PubMed
  8. Li J, et al. 2022. Front Immunol. 13:812164. PubMed
  9. Chen S, et al. 2021. Exp Ther Med. 22:1049. PubMed
  10. Waqas FH, et al. 2022. Clin Transl Med. 12:e931. PubMed
  11. Aarreberg LD, et al. 2019. Mol Cell. 74:801. PubMed
  12. Orzalli MH, et al. 2021. Immunity. 54:1447. PubMed
  13. Guo Y, et al. 2020. J Clin Invest. 130:6301. PubMed
  14. Naji A, et al. 2016. Sci Rep. 6: 26162. PubMed
  15. Niemiec M, et al. 2017. BMC Genomics. 10.1186/s12864-017-4097-4. PubMed
  16. Huang HY, et al. 2018. Int Endod J. 9:1425. PubMed
  17. Ireland D, et al. 2016. Innate Immunity. 10.1177/1753425916672313. PubMed
  18. Orzalli MH, et al. 2018. Mol Cell. 71:825. PubMed
  19. Hoang QTM, et al. 2021. Front Med (Lausanne). 8:676058. PubMed
  20. Tang Y, et al. 2021. Immunity. 54(3):454-467.e6. PubMed
  21. Zhou B, et al. 2021. Cell Rep. 35:108998. PubMed
  22. Iannucci J, et al. 2020. Cell Mol Neurobiol. . PubMed
  23. Sohail A, et al. 2022. PLoS Pathog. 18:e1010219. PubMed
  24. Zhao Q, et al. 2022. iScience. 25:105319. PubMed
  25. de Jesus JCR, et al. 2021. Front Immunol. 12:729182. PubMed
  26. Wang X, et al. 2021. Front Pharmacol. 12:673818. PubMed
  27. Hirschberger S, et al. 2021. EMBO Mol Med. 13:e14323. PubMed
  28. Wu WT, et al. 2021. J Orthop Surg Res. 16:147. PubMed
  29. Wurster S, et al. 2017. Virulence. 8:1708. PubMed
  30. Girsang E, et al. 2021. PeerJ. 9:e11419. PubMed
  31. Marín-Palma D, et al. 2021. Molecules. 26:. PubMed
  32. Luvanda MK, et al. 2021. J Fungi (Basel). 7:. PubMed
Sensitivity
0.5 pg/mL
Standard Range
2.0-125 pg/mL
Materials Not Included
  • Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
  • Deionized (DI) water
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 µm filtered.
  • Wash Buffer (Phosphate-Buffered Saline (PBS) + 0.05% Tween-20, pH 7.4). BioLegend Cat. No. 421601 is recommended.
  • Wash bottle or automated microplate washer
  • Stop Solution (2N H2SO4)
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Plate Shaker
  • Plate Sealer
  • Absorbent paper

Antigen Details

Cell Sources
Monocytes, tissue macrophages, Langerhans cells, dendritic cells, T and B cells, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma, keratinocytes,
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Gene ID
3553 View all products for this Gene ID
UniProt
View information about IL-1beta on UniProt.org

Related FAQs

Do your IL-1β ELISA kits detect pro-IL-1β or the mature form?

The antibodies in our IL-1β kits were generated against the mature form of the protein. However, because the pro-form of the protein contains the mature form, the antibodies should detect both forms.

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 9    Revision Date: 02.16.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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