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Human TBNK Cocktail

Pricing & Availability
Clone
SK7; 5.1H11; B73.1; 2D1; SJ25C1;
Regulatory Status
RUO
Other Names
TBNK 4-Color Cocktail
Isotype
Mouse IgG1, κ (all clones)
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Human_TBNK_Cocktail_1_052516
Human peripheral blood was stained with Human TBNK Cocktail. Middle and bottom panel are gated on CD45+ lymphocytes.
  • Human_TBNK_Cocktail_1_052516
    Human peripheral blood was stained with Human TBNK Cocktail. Middle and bottom panel are gated on CD45+ lymphocytes.
  • Human_TBNK_Cocktail_2_052516
  • Human_TBNK_Cocktail_3_052516
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391501 50 tests 569 CHF
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Description

CD3 (Clone SK7): The CD3/T-cell receptor (TCR) complex is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T cells, NK T cells, and some thymocytes. It plays a role in antigen recognition, signal transduction, and T cell activation.

CD45 (Clone 2D1): It is a type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It functions as a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes or platelets. CD45 regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation.

CD56 (Clone 5.1H11): A single transmembrane glycoprotein, also known as NCAM (neural cell adhesion molecule), Leu-19, or NKH1. It is expressed on NK and NKT cells, but in other cells and tissues as well, such as large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal derived tumors, myelomas, myeloid leukemias, brain cerebellum and cortex, and neuromuscular junctions. CD56 plays a role in homophilic and heterophilic adhesion via binding to itself or heparan sulfate.

CD16 (Clone B73.1): Also known as low affinity IgG receptor III (FcγRIII), it is expressed as two distinct forms (CD16a and CD16b). CD16a (FcγRIIIA) is expressed on the surface of NK cells, activated monocytes, macrophages, a subset of T cells, and placental trophoblasts in humans. CD16b (FcγRIIIB) is expressed specifically on neutrophils. CD16 binds aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC).

CD19 (Clone SJ25C1): Also known as B4, it is a member of the immunoglobulin superfamily expressed on B cells (from pro-B to blastoid B cells, absent on plasma cells) and follicular dendritic cells. CD19 is involved in B cell development, activation, and differentiation. CD19 forms a complex with CD21 (CR2) and CD81 (TAPA-1), and functions as a BCR co-receptor.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
This reagent is a combination of FITC conjugated SK7, PE conjugated 5.1H11, PE conjugated B73.1, PerCP conjugated 2D1, and APC conjugated SJ25C1.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood.

Application Notes

This cocktail is intended to differentiate and determine the frequencies of T-Cells, B-Cells, NK-Cells and NKT-Cells in a single test format using either lyse-wash or lyse-no wash human peripheral blood assays. It may be used as a stand-alone product or as a common marker backbone to which additional antibodies may be added for specific applications.

Note: Isotype controls are not included. Single color compensation controls are not included.

RRID
AB_2616702 (BioLegend Cat. No. 391501)

Antigen Details

Distribution

T-cells, B-cells, NK-cells and NKT-cells.

Cell Type
B cells, NK cells, NKT cells, T cells
Biology Area
Immunology
Antigen References

1. Tedder T, et al. 1994. Immunol. Today 15:437.
2. Bradbury L, et al. 1993. J. Immunol. 151:2915.
3. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press. San Diego.
4. Beverley P, et al. 1981. Eur. J. Immunol. 11:329.
5. Lanier L, et al. 1986. J. Immunol. 137:2501.
6. Schubert J, et al. 1989. In Leucocyte Typing IV (Knapp W, ed) Oxford University Press Oxford pp 711.
7. Palmer BE, et al. 2005. J. Immunol. 175:8415.
8. Schachner M, Martini R. 1995. Trends Neurosci. 18:183.
9. Wood KL, et al. 2005. Clin. Immunol. 117:294.
10. Björkström NK, et al. 2008. J. Immonol. 181:4219.
11. Lanier L, et al. 1991. J. Immunol. 146:4421.
12. Hemperly J, et al. 1990. J. Mol. Neurosci. 2:71.
13. Cremer H, et al. 1994. Nature 367:455.
14. Bradstock KF, et al. 1980. J. Natl. Cancer Inst. 65:33.
15. Csiba A, et al. 1984. Br. J. Cancer 50:699.
16. Tchilian EZ, et al. 2001. J. Immunol. 166:1308.
17. Lee MS, et al. 2004. Int. Immunol. 16:1109.

Gene ID
916 View all products for this Gene ID 4684 View all products for this Gene ID 2214 View all products for this Gene ID 2215 View all products for this Gene ID 5788 View all products for this Gene ID 930 View all products for this Gene ID

Related FAQs

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Other Formats

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Description Clone Applications
Human TBNK Cocktail SK7; 5.1H11; B73.1; 2D1; SJ25C1 FC
Go To Top Version: 1    Revision Date: 05.25.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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