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LEAF™ Purified anti-human IL-1β Antibody

Pricing & Availability
Clone
Poly5174 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-1β, Catabolin, Hematopoietin-1 (H1), IFNβ-inducing factor, Interleukin-β, Osteoclast activating factor (OAF)
Isotype
Goat Polyclonal IgG
Ave. Rating
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Product Citations
publications
Poly5174_LEAF_IL1Beta_Neut_1_081823
Recombinant human IL-1β (Cat. No. 579402) (black circles) induces proliferation of D10.G4.1 cell. LEAF™ purified anti-human IL-1β (clone Poly5174) (purple squares) neutralizes the D10.G4.1 cell proliferation induced by recombinant human IL-1β at 0.03 ng/mL in a dose-dependent manner, whereas the LEAF™ purified goat IgG isotype control (gray triangles) does not have this effect. ND50 range: 2-18 ng/mL.
  • Poly5174_LEAF_IL1Beta_Neut_1_081823
    Recombinant human IL-1β (Cat. No. 579402) (black circles) induces proliferation of D10.G4.1 cell. LEAF™ purified anti-human IL-1β (clone Poly5174) (purple squares) neutralizes the D10.G4.1 cell proliferation induced by recombinant human IL-1β at 0.03 ng/mL in a dose-dependent manner, whereas the LEAF™ purified goat IgG isotype control (gray triangles) does not have this effect. ND50 range: 2-18 ng/mL.
  • Poly5174_LEAF_IL1Beta_WB_2_081823
    Whole cell extracts (15 µg total protein) from indicated cell lines treated with Brefeldin A (Cat. No. 420601) with or without lipopolysaccharide (100 ng/mL) were resolved in 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.5 µg/mL LEAF™ purified anti-human IL-1β (clone Poly5174) for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP Donkey anti-goat IgG. Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control at a 1:10000 dilution.
Cat # Size Price Quantity Check Availability Save
517405 100 µg 445 CHF
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Description

IL-1β is a proinflammatory cytokine essential for host responses and resistance to pathogens, including induction of fever, cell proliferation, differentiation, and apoptosis. IL-1β in humans and mice does not encode a typical signal peptide and, as a result, newly synthesized pro-IL-1β accumulates within the cytoplasm of activated monocytes and macrophages. Conversion of the inactive pro-IL-1β to its mature form requires the proteolytic action of IL-1β-converting enzyme (ICE), also termed caspase-1. Secretion of mature IL-1β from LPS-activated monocytes/macrophages is not a constitutive process. These cells must encounter a secondary stimulus that specifically activates the posttranslational processing events. Moreover, owing to its pro-inflammatory nature, IL-1β is regarded as a tumor-promoting cytokine. In fact, enhanced tumor metastasis and angiogenesis have been observed under the influence of IL-1β. IL-1β is able to facilitate tumor progression in murine models of lung cancer. In addition, upregulation of metastasis and tumor angiogenesis by IL-1β has been associated with increased activity of matrix metalloproteinases and expression of the pro-angiogenic molecule hepatocyte growth factor.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Polyclonal
Host Species
Goat
Immunogen
Recombinant human IL-1β
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.
Preparation
The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Upon receipt, store frozen at -20°C. Make small volume aliquots if needed and avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
Application

Neut - Quality tested
WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by neutralizing proliferation induced by recombinant human IL-1β at 0.03 ng/mL in D10.G4.1 cells. ND50: 2-18 ng/mL. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Detection: The biotinylated Poly5174 is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with the purified H1b-27 antibody (Cat. No. 511601) as the capture antibody and recombinant human IL-1β (Cat. No. 579409) as the standard.

Note: For testing human IL-1β in serum or plasma, BioLegend's LEGEND MAX™ Kits (Cat. No. 437007 & 437008) are specially developed and recommended.

RRID
AB_3083208 (BioLegend Cat. No. 517405)

Antigen Details

Structure
Cytokines/Chemokines
Distribution

IL-1β is expressed in activated monocytes/macrophages.

Function
Interleukin (IL-1β) is a cytokine with a key role in the pathophysiology of local and systemic inflammation. IL-1β induces cytokine, chemokine, proinflammatory molecule secretion, and adhesion molecule expression in diverse cells.
Interaction
Hematopoietic stem and progenitors
Ligand/Receptor
Type I and II IL-1 receptors (IL-1RI and IL-1RII)
Cell Type
Astrocytes, B cells, Dendritic cells, Endothelial cells, Fibroblasts, Langerhans cells, Macrophages, Monocytes, NK cells, T cells
Biology Area
Cell Biology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Stevenson FT, et al. 1992. J Cell Physiol. 152:223-31.
  2. Shi J, et al. 2007. J. Immunol. 179:1245--53
  3. Dinarello CA. 2006. Cancer Metastasis Rev. 25:307-13.
  4. Boost KM, et al. 2008. BMC Cancer. 8:265.
Gene ID
3553 View all products for this Gene ID
UniProt
View information about IL-1beta on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 08.28.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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